Correspondence: E. Papadopoulos, Laboratory of Parasitology and Parasitic Diseases, School of Veterinary Medicine, Faculty of Health Sciences, Aristotle University of Thessaloniki, Thessaloniki, Greece. E-mail: eliaspap@vet.auth.gr Αλληλογραφία: Η. ααδ, Εαστ ααστα α ααστ στ, α τατ,  Εστ εα, Ασττε αεστ Θεσσα, Θεσσα. E-mail: eliaspap@vet.auth.gr J HELLENIC VET MED SOC 2014, 65(2):115-120 ΕΕ 2014, 65(2):115-120 Research article Ερευνητικό άρθρο First report of Besnoitia besnoiti seropositive cattle in Greece Papadopoulos E. 1 , Arsenos G. 2 , Ptochos S. 1 , Katsoulos P. 3 , Oikonomou G. 3 , Karatzia M.A. 2 , Karatzias H. 4 1 Laboratory of Parasitology and Parasitic Diseases, Faculty of Health Sciences, School of Veterinary Medicine, Aristotle University of Thessaloniki, Thessaloniki, Greece. 2 Laboratory of Animal Husbandry, Faculty of Health Sciences, School of Veterinary Medicine, Aristotle University of Thessaloniki, Thessaloniki, Greece. 3 School of Veterinary Science, University of Liverpool, Leahurst, UK. 4 Clinic of Farm Animals, Faculty of Health Sciences, School of Veterinary Medicine, Aristotle University of Thessaloniki Greece. τ ααφ ετττα εδ σε Besnoitia besnoiti στ Εδα ααδ Η. 1 , Ασ Γ. 2 , τ . 1 , ατσ . 3 ,  Γ. 3 , αατ .Α. 2 , αατ . 4 1 Εργαστήριο Παρασιτολογίας και Παρασιτικών Νοσημάτων, Σχολή Επιστημών Υγείας, Τμήμα Κτηνιατρικής, Αριστοτέλειο Πανεπιστήμιο Θεσσαλονίκης, Θεσσαλονίκη. 2 Εργαστήριο Ζωοτεχνίας, Σχολή Επιστημών Υγείας, Τμήμα Κτηνιατρικής, Αριστοτέλειο Πανεπιστήμιο Θεσσαλονίκης, Θεσσαλονίκη. 3 School of Veterinary Science, University of Liverpool, Leahurst, UK. 3 Κλινική Παραγωγικών Ζώων, Σχολή Επιστημών Υγείας, Τμήμα Κτηνιατρικής, Αριστοτέλειο Πανεπιστήμιο Θεσσαλονίκης, Θεσσαλονίκη. ABSTRACT. Aim of this study was to investigate presence of Besnoitia besnoiti infection in indigenous and imported cattle in Greece. A total of 450 bovine blood sera samples from 15 dairy herds of Northern Greece were tested using a commercially avail- able kit to detect specifc anti-B. besnoiti antibodies. Ninety-eight cows were found to be seropositive to B. besnoiti (22%). Serop- ositivity varied among herds from 0% to 35%. Results were analysed using a univariate analysis of variance. A full factorial model was developed, using as independent variables area of sampling (Thrace, Macedonia) and origin of cattle (local, imported). No sig- nifcant effect was found of the variables included into the model (P > 0.05). It is concluded that seropositive dairy cows are present in Northern Greece; however, impact of this parasite on animal production in Greece remains a key topic for further investigation. Keywords: Besnoitia besnoiti, dairy cattle, emerging disease, Greece, protozoan parasite