242 Analytical and Quantitative Cytopathology and Histopathology ® 0884-6812/21/4304-0242/$18.00/0 © Science Printers and Publishers, Inc. Analytical and Quantitative Cytopathology and Histopathology ® OBJECTIVE: In order to reduce complications accom- panied with dental implant restoration, this study strives to prepare a novel sealant and lubricant that can be used in dental implant systems as well as to evaluate its characteristics. STUDY DESIGN: Chitosan (CS), β-glycerophosphate pentahydrate (β-GP), and nano silver (nAg) were used to prepare thermosensitive hydrogel. According to the different volume ratios of CS to β-GP, 3 experimental groups were established, namely 16/4, 13/7, and 10/10 groups. Their morphology, composition, and chemical properties were analyzed via SEM, EDS, and FTIR. In addition, the effect of the hydrogel on the stability of dental implant-abutment connection was investigated by removal torque test combined with dynamic cyclic loading experiment. The maximum fracture load was measured under different lubricating conditions by elec- tronic universal testing machine. The cytotoxicity and in vitro antibacterial effect of the hydrogel were examined respectively by CCK-8 test and the spread plate method. RESULTS: The CS/β-GP/nAg thermosensitive hydro- gel was successfully prepared in this study, which was found to be a porous structure through SEM. The re- moval torque test and the dynamic cyclic loading ex- periment showed that the removal torque of the experi- mental group was greater than that of the control group. Preparation and Properties of Chitosan-Based Thermosensitive Hydrogel and Its Positive Infuence on the Stability in Dental Implant System as Sealant and Lubricant Chunqing Ye, M.M., Xinjie Cai, M.D., Fushi Wang, M.D., Yi Zhou, M.D., Tao Jiang, M.D., and Yining Wang, M.D. From The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei‐MOST) and Key Laboratory of Oral Biomedicine Ministry of Education, School and Hospital of Stomatology, Wuhan University, Wuhan, Hubei Province; and the Department of Pros- thodontics, Hospital of Stomatology, Wuhan University, Wuhan, Hubei Province, China. Chunqing Ye is Student, State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei‐MOST) and Key Laboratory of Oral Biomedicine Ministry of Education, School and Hospital of Stomatology, Wuhan University. Xinjie Cai is Physician, State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei‐MOST) and Key Laboratory of Oral Biomedicine Ministry of Education, School and Hospital of Stomatology, Wuhan University, and Department of Prosthodontics, Hos- pital of Stomatology, Wuhan University. Fushi Wang is Physician, State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei‐MOST) and Key Laboratory of Oral Biomedicine Ministry of Education, School and Hospital of Stomatology, Wuhan University, and Department of Prosthodontics, Hospital of Stomatology, Wuhan University. Yi Zhou is Associate Professor, State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei‐MOST) and Key Laboratory of Oral Biomedicine Ministry of Education, School and Hospital of Stomatology, Wuhan University, and Department of Prosthodontics, Hospital of Stomatology, Wuhan University. Tao Jiang is Professor, Department of Prosthodontics, Hospital of Stomatology, Wuhan University. Yining Wang is Professor, Department of Prosthodontics, Hospital of Stomatology, Wuhan University. Chunqing Ye and Xinjie Cai contributed equally. Address correspondence to: Tao Jiang, M.D., and Yining Wang, M.D., Department of Prosthodontics, Hospital of Stomatology, Wuhan University, Luoyu Road 237, Hongshan District, Wuhan 430079, Hubei Province, China (jiangtao2006@whu.edu.cn and wang.yn@ whu.edu.cn). Financial Disclosure: The authors have no connection to any companies or products mentioned in this article.