Research Article Antioxidant Activity and Antibacterial Effects on Clinical Isolated Streptococcus suis and Staphylococcus intermedius of Extracts from Several Parts of Cladogynos orientalis and Their Phytochemical Screenings Pongtip Sithisarn, 1 Piyanuch Rojsanga, 2 Patchima Sithisarn, 3 and Sumet Kongkiatpaiboon 4 1 Department of Pharmacognosy, Faculty of Pharmacy, Mahidol University, Bangkok 10400, Tailand 2 Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Mahidol University, Bangkok 10400, Tailand 3 Department of Veterinary Public Health, Faculty of Veterinary Medicine, Kasetsart University, Nakhon Pathom 73140, Tailand 4 Drug Discovery and Development Center, Tammasat University, Rangsit Campus, Pathum Tani 12121, Tailand Correspondence should be addressed to Pongtip Sithisarn; pongtip.sit@mahidol.ac.th Received 12 May 2015; Revised 13 July 2015; Accepted 27 July 2015 Academic Editor: Vincenzo De Feo Copyright © 2015 Pongtip Sithisarn et al. Tis is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Te in vitro antioxidant and antibacterial assays against clinically isolated Streptococcus suis and Staphylococcus intermedius of the extracts prepared by decoction and ethanolic refux of diferent parts of Chettaphangki (Cladogynos orientalis Zipp. ex Span), including the leaves, roots, and stems, using 1,1-diphenyl-2-picrylhydrazyl (DPPH) scavenging assay and disc difusion method were conducted. Quantitative analysis of total phenolic and total favonoid contents in the extracts using spectrophotometric methods was also performed. Finally, phytochemical screening by thin layer chromatography (TLC) and high performance liquid chromatography (HPLC) was conducted. Leaf ethanolic refux extract (100 g) contained the highest total phenolic and total favonoid contents of 7.21 ± 0.28 g gallic acid equivalent (GAE) and 11.51 ± 2.02 g rutin equivalent (RE), respectively. Chettaphangki extracts promoted low antioxidant activity with EC 50 values in the range of 0.27–0.48 mg/mL. Extracts and fractions from the roots and stems of this plant promoted low to intermediate antibacterial activity against S. intermedius with the inhibition zones between 7 and 14 mm. Te chromatographic data suggested that the leaf extracts of C. orientalis contained rutin while the root and stem extracts contained scopoletin and chettaphanin I. Rutin promoted strong antioxidant activity while chettaphanin I showed low antibacterial activity against Staphylococcus intermedius. 1. Introduction Cladogynos orientalis, colloquially known in Tai as Chet- taphangki, is a plant in Euphorbiaceae family which has been used in traditional medicine as antifatulent, antistom- achache, and tonic agent [1]. Te roots of this plant are assigned in National List of Essential Medicines 2013, as the component in the formulation to treat fatulence and colic [1]. Previous report suggested that the extract from the whole plant of C. orientalis promoted antidengue virus efect using MTT assay [2]. Te leaf extract also promoted efective inhibition of human hepatocarcinoma (HepG2) [3]. It was previously reported that terpenoid compounds including ent-halimane diterpenes; chettaphanin I and chettaphanin II, sesquiterpenes; 8-hydroxy-alpha-guaiene, spathulenol, cyperenoic acid, and triterpenes; and taraxerol and ace- toxyaleuritolate were the major components in C. orien- talis [4–6]. Some unusual aromatic diglycosides includ- ing 4  -O-galloyl-violutoside and 4  -O-galloyl-benzyl-O-- L-rhamnopyranosyl-(1 → 6)--D-glucopyranoside and some favonoids such as isovitexin and apigenin glycosides were found in the aerial part of this plant [7]. Moreover, the leaves Hindawi Publishing Corporation Evidence-Based Complementary and Alternative Medicine Volume 2015, Article ID 908242, 8 pages http://dx.doi.org/10.1155/2015/908242