Research Article IncrementofLysosomalBiogenesisbyCombinedExtractsofGum Arabic, Parsley, and Corn Silk: A Reparative Mechanism in Mice Renal Cells Aya Helmy , 1 Mohamed El-Shazly, 2,3 Nesreen Omar , 4 Mohamed Rabeh, 1,5 Usama Ramadan Abdelmohsen, 6,7 Reham Tash, 8,9 Mohammad Alaraby Salem, 10 Ahmed Samir, 10 Ali Elshamy, 5 and Abdel Nasser B. Singab 2 1 Department of Pharmacognosy, Faculty of Pharmacy, Modern University for Technology and Information (MTI), Cairo 11571, Egypt 2 Department of Pharmacognosy, Faculty of Pharmacy, Ain Shams University, Organization of African Unity Street, Abassia, Cairo 11566, Egypt 3 Department of Pharmaceutical Biology, Faculty of Pharmacy and Biotechnology, German University in Cairo, Cairo 11835, Egypt 4 Department of Biochemistry, Faculty of Pharmacy, Modern University for Technology and Information (MTI), Cairo 11571, Egypt 5 Department of Pharmacognosy, Faculty of Pharmacy, Cairo University, Cairo 11562, Egypt 6 Department of Pharmacognosy, Faculty of Pharmacy, Minia University, Minia 61519, Egypt 7 Department of Pharmacognosy, Faculty of Pharmacy, Deraya University, Universities Zone, New Minia City 61111, Egypt 8 Department of Anatomy and Embryology, Faculty of Medicine, Ain Shams University, Abassia, Cairo 11566, Egypt 9 Department of Anatomy and Embryology, Faculty of Medicine, King Abdulaziz University, Rabigh 25724, Saudi Arabia 10 Department of Pharmaceutical Chemistry, Faculty of Pharmacy, October University of Modern Sciences and Arts (MSA), Giza 12585, Egypt Correspondence should be addressed to Nesreen Omar; dr.n.nabil@gmail.com and Abdel Nasser B. Singab; dean@ pharma.asu.edu.eg Received 1 February 2020; Accepted 2 June 2020; Published 11 July 2020 Academic Editor: Letizia Angiolella Copyright © 2020 Aya Helmy et al. is is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Gum Arabic (GA), parsley, and corn silk have been traditionally used for renal failure patients worldwide. is study aimed at probing the mechanism of the combined extracts, namely, GA (3 g/kg/day), parsley (1 g/kg/day), and corn silk (200 mg/kg/ day), as nephroprotective agents in mice after amikacin (1.2g/kg) single dose through exploration of their action on G-protein coupled receptors (GPR) 41 and 43 and the ensuing lysosomal biogenesis. Western blotting was employed for renal levels of bcl-2-associated X protein (BAX) and cytosolic cathepsin D; cell death markers, nuclear transcription factor EB (TFEB), and lysosomal associated membrane protein-1 (LAMP-1); and lysosomal biogenesis indicators. Liquid chromatography–mass spectrometry (LC-MS) and docking were also employed. After amikacin treatment, BAX and cathepsin D levels were upregulated while LAMP-1 and nuclear TFEB levels were inhibited. e combined extracts inhibited BAX and cytosolic cathepsin D but upregulated LAMP-1 and nuclear TFEB levels. Docking confirmed GPR modulatory signaling. e combined extracts showed GPR signal modulatory properties that triggered lysosome synthesis and contributed to reversing the adverse effects of amikacin on renal tissues. Hindawi Evidence-Based Complementary and Alternative Medicine Volume 2020, Article ID 8631258, 12 pages https://doi.org/10.1155/2020/8631258