S 1
Supporting Information
Oxo-Ester Mediated Native Chemical Ligation on Microarrays:
An Efficient and Chemoselective Coupling Methodology
Martin J. Weissenborn,
a
Roberto Castangia,
a
Johannes W. Wehner,
b
Robert
Šardzík,
a
Thisbe K. Lindhorst*
b
and Sabine L. Flitsch*
a
a
School of Chemistry & Manchester Interdisciplinary Biocentre, The University of Manchester, 131 Princess
Street, Manchester, M17DN (UK). Fax: 44- 161-275-1311; Tel: -44- 161-306-5172; E-mail:
sabine.flitsch@manchester.ac.uk
b
Otto Diels Institute of Organic Chemistry, Christiana Albertina University of
Kiel, Otto-Hahn-Platz 3/4, 24098 Kiel, Germany. Fax: 49-431-880-7410; E-mail: tklind@oc.uni-kiel.de
1. General methods ........................................................................................ 2
2. Peptide synthesis ........................................................................................ 3
3. Saccharide synthesis .................................................................................. 4
4. Coupling on Self-Assembled Monolayers (SAMs) ................................. 17
5. MALDI-ToF MS spectra ......................................................................... 20
6. Surface Plasmon Resonance (SPR) Analysis .......................................... 38
Electronic Supplementary Material (ESI) for Chemical Communications
This journal is © The Royal Society of Chemistry 2012