Agricultural Sciences, 2017, 8, 582-590 http://www.scirp.org/journal/as ISSN Online: 2156-8561 ISSN Print: 2156-8553 DOI: 10.4236/as.2017.87044 July 25, 2017 Pre-Inoculation with Bradyrhizobium spp. in Industrially Treated Soybean Seeds Fernanda Brunetta Godinho Anghinoni 1* , Alessandro Lucca Braccini 2 , Carlos Alberto Scapim 2 , Guilherme Anghinoni 1 , Glaucia Cristina Ferri 1 , Andréia Kazumi Suzukawa 1 , Telmo Antonio Tonin 2 1 Postgraduate Program in Agronomy, State University of Maringa, Maringa, Brazil 2 Department of Agronomy, State University of Maringa, Maringa, Brazil Abstract The objective of this study was to compare the effect of anticipated and tradi- tional inoculation of Bradyrhizobium spp. in industrially treated soybean seeds. The experiment was conducted in a randomized complete block design, with 10 treatments and 4 replications. Different combinations of insecticides and fungicides of industrial treatment of seeds, traditional inoculation and long- term (LT) inoculation, as well as different pre-inoculation periods performed at 0, 5 and 10 days before soybean sowing were evaluated. A treatment con- taining only nitrogen mineral fertilization and also a control treatment were included. The characteristics number of nodules per plant, seeds Nitrogen content, number of pods per plant, mass of thousand seeds and grain yield were evaluated. Results indicated that LT pre-inoculation combined with flu- dioxonil and thiamedoxan allowed seed storage up to 10 days without affect- ing soybean yield components. Keywords Nitrogen, Nodulation, Insecticide, Fungicide, Yield 1. Introduction Soybean crop established a large importance on the economic scenario for differ- ent countries, especially in the last decades [1], because of its high protein content, about 35% - 40%, playing an important role in human and animal nutrition [2]. Nitrogen stands out as an important nutrient, participating in the composi- tion of amino acids, proteins, chlorophyll and many essential enzymes, stimu- lating roots and shoots growth. There is a high concern about N use in agricul- ture, since losses by volatilization, denitrification, erosion and leaching that How to cite this paper: Anghinoni, F.B.G., Braccini, A.L., Scapim, C.A., Anghinoni, G., Ferri, G.C., Suzukawa, A.K. and Tonin, T.A. (2017) Pre-Inoculation with Bradyr- hizobium spp. in Industrially Treated Soy- bean Seeds. Agricultural Sciences, 8, 582- 590. https://doi.org/10.4236/as.2017.87044 Received: May 1, 2017 Accepted: July 22, 2017 Published: July 25, 2017 Copyright © 2017 by authors and Scientific Research Publishing Inc. This work is licensed under the Creative Commons Attribution International License (CC BY 4.0). http://creativecommons.org/licenses/by/4.0/ Open Access