Indra G, Maragathavalli G, Rajeshkumar S. Preparation Of Mouthwash Using Triphala Aqua - Ethanolic Extract - A Comparative Study On Antimicrobial And Cytotoxic Effects. Int J Dentistry Oral Sci. 2021;8(8):3648-3651. 3648 OPEN ACCESS https://scidoc.org/IJDOS.php Preparation Of Mouthwash Using Triphala Aqua - Ethanolic Extract - A Comparative Study On Antimicrobial And Cytotoxic Effects Research Article International Journal of Dentistry and Oral Science (IJDOS) ISSN: 2377-8075 *Corresponding Author: Maragathavalli G, Professor, Department of Oral Medicine and Radiology, Saveetha Dental College and Hospital, Saveetha Institute of Medical and Technical Sciences (SIMATS), Chennai - 77, India. Tel: +91 9445171146 E-mail: drgopalvalli@ gmail.com Received: April 15, 2021 Accepted: July 22, 2021 Published: August 03, 2021 Citation: Indra G, Maragathavalli G, Rajeshkumar S. Preparation Of Mouthwash Using Triphala Aqua - Ethanolic Extract - A Comparative Study On Antimicrobial And Cytotoxic Effects. Int J Dentistry Oral Sci. 2021;8(8):3648-3651. doi: http://dx.doi.org/10.19070/2377-8075-21000746 Copyright: Maragathavalli G © 2021. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution and reproduction in any medium, provided the original author and source are credited. Indra G 1 , Maragathavalli G 2* , Rajeshkumar S 3 1 Post graduate resident, Department of Oral Medicine and Radiology, Saveetha Dental College and Hospital, Saveetha Institute of Medical and Techni- cal Sciences (SIMATS), Chennai - 77, India. 2 Professor, Department of Oral Medicine and Radiology, Saveetha Dental College and Hospital, Saveetha Institute of Medical and Technical Sciences (SIMATS), Chennai - 77, India. 3 Associate Professor, Department of Pharmacology, Saveetha Dental College and Hospital, Saveetha Institute of Medical and Technical Sciences (SI- MATS), Chennai - 77, India. Introduction In India, the traditional medicines such as AYUSH (Ayurveda, Yoga and Naturopathy, Unani, Siddha and Homeopathy) have been in practice for centuries and focus on healing of the mind and body and in treating different ailments.[1] Triphala is an ayurvedic polyherbal medicine mainly consisting of three ingredients namely, Amalaki (Phyllanthus emblica), Bib- hitaki (Terminalia bellirica), and Haritaki (Terminalia chebula) in equal proportions.[2] The phytochemicals present in it are com- ponents rich in gallic acid, tannins, chebulagic acid, ellagic acid, phenols and glycosides.[3] Triphala is reported to be an effec- tive antibacterial, antifungal, antioxidant, chemopreventive, anti- diabetic, radioprotective, antimutagenic, analgesic, anticancer and antipyretic agents. [3-11] In dentistry, triphala are proven to have good anticariogenic ef- fcacy, used as a root canal irrigant, anti-collagenase activity, an- tifungal, antioxidant and as mouth rinse.[12] Triphala have also shown to be a good denture cleanser. It is because of the gallic acid component in the triphala accountable for the anticandidal effcacy.[13] Triphala is commercially available as choorna and tablets. Maxi- mum dosage of Triphala extract is 2000 mg per day. Concentrate tablets of Triphala contain around 500 mg to 750 mg depending on the manufacturer.[12, 14] Abstract Objective: The objective of the study was to prepare mouthwash using triphala aqua-ethanolic extract and to compare and evaluate the antimicrobial and cytotoxic effects. Materials and method: This study includes triphala churna powder which is the key ingredient and absolute ethanol as the solvent for preparing the mouthwash and subjected to antimicrobial effcacy and cytotoxic effects. For antimicrobial activity, four microorganisms, S.aureus, S. mutans, E. faecalis and C. albicans are used and checked for zones of inhibition. For cyto- toxic assay, brine shrimp is used to check for cytotoxicity after 24 hours. Result: The cytotoxicity assessment of the number of live nauplii after 24 hours revealed that there were at least 8 nauplii alive in each group. Zone of inhibition for 25μL showed 40% inhibition of S.aureus, in 50μL the S.aureus showed 32% inhibition and in 100μL it showed 30% inhibition. Conclusion: Triphala aqua-ethanolic mouthwash has proven to have excellent antimicrobial properties and hence can be used in dentistry. Elaborate studies should be done to evaluate the toxic potential before using in human beings. Keywords: Triphala; Antimicrobial Activity; Cytotoxicity; Mouthwash; Plant Extract.