*Correspondence: kit.w@ubru.ac.th; +66-85-6325717 (Received: 10 November 2018; accepted: 22 December 2018) Citaton: Kit Wirunpan, Sakesan Chinwang, Nareerat Chaikong and Charida Pukahuta, Increasing Nutritonal Contents of Cassava Starch Wastes Using Pleurotus ostreatus (Jacq.) P.Kumm. and Lentnus squarrosulus Mont., J Pure Appl Microbiol., 2019; 13(1):117- 125 doi: 10.22207/JPAM.13.1.12 © The Author(s) 2019. Open Access. This artcle is distributed under the terms of the Creatve Commons Atributon 4.0 Internatonal License which permits unrestricted use, sharing, distributon, and reproducton in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creatve Commons license, and indicate if changes were made. Wirunpan et al. J Pure Appl Microbiol, 13(1), 117-125 | March 2019 Artcle 5363 | htps://dx.doi.org/10.22207/JPAM.13.1.12 Print ISSN: 0973-7510; E-ISSN: 2581-690X ReseARCh ARtiCle www.microbiologyjournal.org 117 Journal of Pure and Applied Microbiology Increasing Nutritonal Contents of Cassava Starch Wastes Using Pleurotus ostreatus (Jacq.) P.Kumm. and Lentnus squarrosulus Mont. Kit Wirunpan 1 *, Sakesan Chinwang 2 , Nareerat Chaikong 1 and Charida Pukahuta 1 1 Department of Biological Science, Faculty of Science, Ubon Ratchathani University, Ubon Ratchathani 34190, Thailand. 2 Department of Agriculture, Faculty of Agriculture, Ubon Ratchathani Rajabhat University. Ubon Ratchathani 34000, Thailand. Abstract This research studied the increasing of nutritonal content in cassava starch fermented with P. ostreatus (Jacq.) P.Kumm. and L. squarrosulus Mont. The cassava starch waste was supplemented with maize grain, paddy rice, rice bran, broken rice, and soybean meal at 0, 10, 20, and 30%. Solid state fermentaton was carried out for 28 days. The results showed that highest reduced sugar at 86.09 g/L was found in cassava starch waste 70% + rice broken 30% fermented by L. squarrosulus Mont. The highest protein content at 127.92 g/L was found in cassava starch waste 90% + soybean meal 10% fermented with cassava starch waste 70% + rice broken 30% for 14 days. It is concluded from this experiment that P. ostreatus (Jacq.) P.Kumm. and L. squarrosulus Mont. can increase nutritonal content in several feed mixtures. Keywords: Cassava Starch Wastes, Pleurotus ostreatus (Jacq.) P.Kumm., nutritonal content, Lentnus squarrosulus Mont.