DOI: http://dx.doi.org/10.18185/eufbed.22429 201 EÜFBED - Fen Bilimleri Enstitüsü Dergisi Cilt-Sayı: 8-2 Yıl: 2015 201-212 *Sorumlu Yazar: mcankaya@erzincan.edu.tr EFFECT OF SOME DRUGS ON SHEEP LIVER CARBONIC ANHYDRASE I AND II BAZI İLAÇLARIN KOYUN KARACİĞERİ KARBONİK ANHİDRAZ I VE II ÜZERİNDEKİ ETKİSİ Mehmet KUZUCU, Murat CANKAYA* 1 Erzincan Üniversitesi Fen Edebiyat Fakültesi Biyoloji Bölümü Erzincan, TÜRKİYE Geliş Tarihi (Received): 30/01/2015 Kabul Tarihi (Accepted): 10/06/2015 ABSTRACT Effects of clindamycin, dexketoprofen trometamol, benzylpenicillin (penicillin G) and phenoxymethylpenicillin (penicillin V) were examined on purified sheep carbonic anhydrase I and II (SCAI, SCA-II). In our study, we were chosen sheep liver tissue; because the liver is responsible for detoxification of xenobiotics. The CA isozyme have a very important duties on organisms, the effect of xenobiotics on the CA enzyme was reported. It was observed these drugs exhibited the inhibition effects against liver carbonic anhydrase isozymes I and II in our study. SCA I and SCA II isozymes were purified with about a specific activity of 1108.51 EU/mg and 5706.67 EU/mg, 120.62 and 620.96 purification fold by using Sepharose-4B-L tyrosine-sulfanilamide affinity gel chromatography. Purity of the SCA I and SCA II isozyme was checked by SDS-PAGE technique and subunit molecular masses of the SCA I was found as 29 kDa and SCA II found as 31 kDa. The usage of clindamycin, dexketoprofen trometamol, benzylpenicillin (penicillin G) and phenoxymethylpenicillin (penicillin V) in sheep liver especially during necrotic hepatitis disease is important for scientific researches. Many antibiotic and antihistamine may have dangerous effect to sheep liver. Also, it is important to study the inhibition effects of these drugs on SCA I and II isozymes. Key words: Liver, Antibiotics, Inhibition. ÖZET Koyun karaciğerinden saflaştırılmış karbonik anhidraz I ve II (SCAI , SCA-II) üzerine; klindamisin, deksketoprofen trometamol, benzilpenisilin (penisilin G) ve fenoksimetilpenisilin (penisilin V)’in etkileri incelenmiştir. Çalışmamızda koyun karaciğer dokusunu seçtik, çünkü karaciğer ksenobiyotiklerin detoksifikasyonundan sorumludur. Karbonik anhidraz izoenzimlerinin organizmalarda çok önemli görevleri vardır, ksenobiyotiklerin karbonik anhidraz üzerindeki etkileri rapor edilmiştir.