Research Article Magnetic Fluid Hyperthermia of Rodent Tumors Using Manganese Perovskite Nanoparticles Larissa Bubnovskaya, 1 Anatolij Belous, 2 Sergej Solopan, 2 Antonina Kovelskaya, 1 Lyudmila Bovkun, 1 Alexander Podoltsev, 3 Igor Kondtratenko, 3 and Sergej Osinsky 1 1 R.E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology, National Academy of Sciences of Ukraine, Vasilkovskaya Street 45, Kiev 03022, Ukraine 2 V.I. Vernadsky Institute of General and Inorganic Chemistry, National Academy of Sciences of Ukraine, Prospekt Palladina 32/34, Kiev 03142, Ukraine 3 Institute of Electrodynamic, National Academy of Sciences of Ukraine, Prospekt Pobedy 56, Kiev 03057, Ukraine Correspondence should be addressed to Sergej Osinsky; osinskysp12@ukr.net Received 30 May 2014; Accepted 18 July 2014; Published 14 August 2014 Academic Editor: Fabien Grasset Copyright © 2014 Larissa Bubnovskaya et al. Tis is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Purpose. To test the antitumor activity of magnetic fuid (MF) on the basis of substituted lanthanum-strontium manganite nanoparticles combined with alternating magnetic feld (AMF) in experiments with transplanted tumors. Materials and Methods. MF with a size of nanoparticles of 30–40 nm in aqueous agarose solution was investigated. Te ability of MF to heat tumor under AMF (300 kHz, 7.7 kA/m) was tested in vivo with rodent tumors (Guerin carcinoma, Walker-256 carcinosarcoma, and Lewis lung carcinoma (3LL)). Results. Single administration of MF into the tumor at a dose of 150 mg/kg (rats) or 200 mg/kg (mice) followed by AMF within 20–30 min (treatment was repeated 3-4-fold) has resulted in the complete regression of tumor in the 35% of rats and 57% of mice. Administration of MF alone or action of AMF alone has not resulted in tumor growth inhibition. Te chemomodifying efect of nanohyperthermia was determined, in particular for cisplatinum: thermal enhancement ratio was 2.0. It was also observed that nanohyperthermia has resulted in the absence of 3LL metastases in 43% of mice. Conclusions. MF on the basis of lanthanum- strontium manganite may be considered as an efective inductor of tumor local hyperthermia. 1. Introduction Te hyperthermia (HT) of human malignant tumor is used in the combined antitumor therapy and demonstrates sig- nifcant modifying efect that results in the improvement of patients’ survival [1–3]. At the same time technical problems of microwave HT, that is, a main method for tumor heating, do not allow receiving the maximal homogenous heating of tumor and cause the defnite side efects. One of the approaches to resolve this problem is a creation of magnetic fuids (MF) on the basis of ferromagnetic nanoparticles which are able to heat the tumor tissue under external alternating magnetic feld (AMF), that was shown in the pioneer work of Jordan et al. [4]. Te use of nanosized magnetic particles, which are able to generate heat under AMF, ofers much extensive scope for the use of HT in the therapy of malignant tumors [5–7]. Te efective application of magnetic nanoparticles was shown in the combination with AMF in the experiment with transplanted tumors both in the regime of thermal ablation and thermochemotherapy [8, 9]. Te nanoparticles of ferromagnetic materials have to meet various requirements; in particular, they must be slightly agglomerated, small sized, single-domained, easily excretable from the body, able to demonstrate high values of the SLP (specifc loss power), and able to exhibit superpara- magnetic properties that would not lead to the interaction of particles afer removing the magnetic feld [5, 6, 10]. Te studies of MF on the basis of magnetite nanoparticles, that is, Fe 3 O 4 , that has the high level of biocompatibility, have received signifcant distribution since the frst publication of Jordan et al. [4]. Among many of publications we would Hindawi Publishing Corporation Journal of Nanoparticles Volume 2014, Article ID 278761, 9 pages http://dx.doi.org/10.1155/2014/278761