www.wjpps.com │ Vol 10, Issue 10, 2021. │ ISO 9001:2015 Certified Journal │ 19 Pimpale et al. World Journal of Pharmacy and Pharmaceutical Sciences DEVELOPMENT AND VALIDATION OF AN RP-HPLC METHOD FOR ANALYSIS OF CRISABOROLE Amol Pimpale* 1 , Sheetal Gondkar 2 and Smita Aher 3 1 Department of Quality Assurance Techniques, R. G, Sapkal College of Pharmacy, Anjaneri, Nashik-422212. 2 Department of Pharmaceutics, R. G, Sapkal College of Pharmacy, Anjaneri, Nashik-422212. 3 Department of Pharmaceutical Chemistry, R. G, Sapkal College of Pharmacy, Anjaneri, Nashik-422212. ABSTRACT A simple, sensitive and accurate RP-HPLC method has been developed for the determination of Crisaborole in bulk formulation. The λmax of the Crisaborole was found to be 241nm in 10 mM Ammonium acetate buffer: ACN [60:40 % v/v]. pH 4.5. The method shows high sensitivity with linearity 5 to 25μg/ml (regression equation: y = 316606x - 427583; r 2 = 0.9992). The various parameters according to ICH guidelines and USP are followed for validating and testing of this method. The Detection limit and quantitation limit were found to be 0.1094 μg ml –1 and 0.3316 μg ml–1 respectively. The results demonstrated that the procedure is accurate, specific and reproducible (RSD <2%), and also being simple, cheap and less time consuming and appropriate for the determination of Crisaborole in bulk. KEYWORDS: Crisaborole, HPLC method. INTRODUCTION Crisaborole is a novel oxaborole endorsed by FDA on December 14, 2016 as Eucrisa, a skin treatment of for mellow to direct atopic dermatitis. By synthetically Crisaborole is 4-[(1- hydroxy-1, 3-dihydro-2, 1-benzoxaborol-5-yl)oxy]benzonitrile and having atomic equation is C14H10BNO3. The construction of Crisaborole is appearing in fig 1. WORLD JOURNAL OF PHARMACY AND PHARMACEUTICAL SCIENCES SJIF Impact Factor 7.632 Volume 10, Issue 10, 19-27 Research Article ISSN 2278 – 4357 *Corresponding Author Amol Pimpale Department of Quality Assurance Techniques, R. G, Sapkal College of Pharmacy, Anjaneri Nashik- 422212. Article Received on 30 July 2021, Revised on 20 August 2021, Accepted on 10 Sept. 2021 DOI: 10.20959/wjpps202110-20098