498 https://www.id-press.eu/mjms/index Scientifc Foundation SPIROSKI, Skopje, Republic of Macedonia Open Access Macedonian Journal of Medical Sciences. 2020 Jun 20; 8(A):498-501. https://doi.org/10.3889/oamjms.2020.3925 eISSN: 1857-9655 Category: A - Basic Sciences Section: Immunology The Role of Collagen Scaffold and Stromal Vascular Fraction on Healing Process in Growth Plate Injury (SOX9 and Histological Examination) Panji Sananta 1,2 *, Mohamad Ibnu Imadudin 2 , I. Gede Made Oka Rahaditya 2 , Marvin Anthony Putera 2 , Sri Andarini 3 , Umi Kalsum 4 , Respati Suryanto Dradjat 2 1 Doctoral Program of Medical Sciences, Faculty of Medicine, Universitas Brawijaya, Jalan Jaksa Agung Suprapto No. 2, Klojen, Kota Malang, Jawa Timur 65112, Indonesia; 2 Department of Orthopaedic and Traumatology, Faculty of Medicine, Universitas Brawijaya, Jalan Jaksa Agung Suprapto No. 2, Klojen, Kota Malang, Jawa Timur 65112, Indonesia; 3 Department of Public Health, Faculty of Medicine, Universitas Brawijaya, Jalan Veteran No. 2, Klojen, Kota Malang, Jawa Timur 65145, Indonesia; 4 Department of Pharmacology, Faculty of Medicine, Universitas Brawijaya, Jalan Veteran No. 2, Klojen, Kota Malang, Jawa Timur 65145, Indonesia AQ5 Abstract BACKGROUND: Pediatric skeletal trauma presents a distinct approach to its treatment because of its unique auses and possibility of its troublesome complications. One of its major complications is growth plate injury which may disturb the longitudinal growth of child’s bone. AIM: In this study, the author combined stromal vascular fraction (SVF) and collagen scaffold as biomaterial for future treatment of physeal injury in skeletally immature patients using SOX9 and histological examination as a marker. METHODS: The study was conducted experimentally on Rattus norvegicus growth plate based on Erickson study, and the SVF was from R. norvegicus fat tissue with ZUK method. Histological examination was evaluated with 8 times magnifcation and SOX9 from the growth plate was measured with enzyme-linked immunosorbent assay. RESULTS: Histological examination showed that the best result was obtained in SVF + collagen group judging by the bony bridge diameter. The result was differed signifcantly from the positive control group with p < 0.05 in Tukey test. The result from SOX9 level measurement shows that all of the treatment groups SOX9 level almost the same as normal value (negative control group) and it is signifcantly differ from the positive control group with p < 0.05 in Mann–Whitney U-test. CONCLUSIONS: This study showed that the combination of collagen and SVF had a great effect on healing process in growth plate injury. Introduction Injuries incurred by skeletally immature patients are unique both in their causes and gravity of their consequences. Physes, or growth plates, are cartilaginous regions at the ends of children’s long bones which act as primary sites of bone elongation. Growth plate injury may result from trauma, infection, metabolic abnormalities, or malignancy. The major concern with growth plate injury is the damaged cartilage in growth plate that could be replaced by bony repair tissue, forming a “bony bridge.” Depending on the size and location of the injury within the growth plate, the bony bridge may cause asymmetric growth arrest with subsequent angular deformity or complete cessation of longitudinal growth. The latter is a devastating outcome for children that have not yet reached their full height. The current treatment involves surgical resection of the bony bridge and replacement with an interpositional material to preserve normal growth in the remaining growth plate. Bony bridge reformation and additional growth effects, however, remain major complications of bar excision. The current management, surgical or non- surgical, has signifcant limitations and may result in further morbidity in the form of additional surgeries, further development of growth arrest, or progression of angular limb deformities. As such, there is a dire need to develop new treatment strategies for growth plate injury that not only prevents bony bridge formation but also leads to regeneration of healthy growth plate cartilage, Edited by: Mirko Spiroski Citation: Sananta P, Imadudin MI, Rahaditya IGMO, Putera MA, Andarini S, Kalsum U, Dradjat RS. The Role of Collagen Scaffold and Stromal Vascular Fraction on Healing Process in Growth Plate Injury (SOX9 and Histological Examination). Open Access Maced J Med Sci. 2020 Jun 20; 8(A):498-501. https://doi. org/10.3889/oamjms.2020.3925 Keywords: Collagen scaffold; Histological examination; Physeal injury; SOX9; Stromal vascular fraction *Correspondence: Panji Sananta, Doctoral Program of Medical Sciences, Faculty of Medicine, Universitas Brawijaya and Department of Orthopaedic and Traumatology, Faculty of Medicine, Universitas Brawijaya, Jalan Jaksa Agung Suprapto No. 2, Klojen, Kota Malang, Jawa Timur 65112, Indonesia. Phone: +6282233600946. E-mail: panjisananta@ub.ac.id Received: 28-Oct-2019 Revised: 25-Mar-2020 Accepted: 29-Mar-2020 Copyright: © 2020 Panji Sananta, Mohamad Ibnu Imadudin, I. Gede Made Oka Rahaditya, Marvin Anthony Putera, Sri Andarini, Umi Kalsum, Respati Suryanto Dradjat Funding: This research did not receive any fnancial support Competing Interests: The authors have declared that no competing interests exist Open Access: This is an open-access article distributed under the terms of the Creative Commons Attribution- NonCommercial 4.0 International License (CC BY-NC 4.0) AQ5