ODONTO Dental Journal. Volume 7. Nomor 1. Juli 2020 Mujayanto/ Shafia/ Feranisa 53 BAY LEAF (SYZYGIUM POLYANTHUM) EXTRACT EFFECT ON IL-10 EXPRESSION IN ORAL ULCER Rochman Mujayanto*, Arina Shafia**, Anggun Feranisa*** ABSTRACT Background: Traumatic ulcers were pathological conditions characterized by loss of epithelial and basal layers resulting from tissue surface excavation caused by trauma. Ulcer healing process consisted of inflammatory, proliferation, and maturation phases. One of the cytokines that play a role in the anti-inflammatory process was IL-10 which worked by balancing the excessive amount of pro-inflammatory cytokines. Bay leaf contained flavonoids which had anti-inflammatory effects. This study’s objective is to determine the effect of bay leaf extract gel on IL-10 expression in the healing process of traumatic ulcers. Method: This study was an analytical study of true experimental laboratories post-test only, consisting of four groups and a sample of 20 male Wistar rats. Mucous ulcers on rat lip were treated with bay leaf extract gel until the 3rd and 5th days. Rat mucosal tissue would be made histological preparations with immunohistochemical staining and calculated IL-10 expression using the Hotspot method. Data obtained then analyzed using Independent T-Test. Result: Results explained that there was a significant increase in IL-10 expression in the group treated using 10% bay leaf extract gel than the control group on 3 rd and 5 th day, which was marked by a significant p <0.05. Conclusion: The conclusion obtained was 10% bay leaf extract (Syzygium polyanthum) Gel effective in increasing IL-10 expression in the healing of the mucous traumatic ulcer of the mouth of the Wistar rat. PENDAHULUAN Ulkus merupakan keadaan patologis yang ditandai dengan hilangnya lapisan epitel dan lapisan basalis akibat dari ekskavasi permukaan jaringan 1 . Penyebab umum terjadinya ulkus pada membran mukosa mulut adalah trauma, baik itu trauma mekanik, kimia, elektrik, atau suhu. Ulkus ini disebut dengan ulkus traumatikus atau traumatic ulcer 2 . Proses penyembuhan ulkus terdiri dari fase inflamasi, proliferasi, serta maturasi. Fase-fase ini terjadi saling tumpang tindih (overlapping), dan berlangsung sejak terjadinya luka, sampai tercapainya resolusi luka 2 . Di fase awal inflamasi, terlihat peningkatan sitokin pro-inflamasi yang menyebabkan aktifnya sel imun di daerah infeksi. Sitokin pro-inflamasi dalam jumlah berlebihan dapat merusak dinding vaskular dan mengakibatkan disfungsi organ. Sitokin anti inflamasi seperti IL-10 serta TGF-β dilepaskan untuk mengimbangi jumlah sitokin pro-inflamasi yang berlebihan 3 . IL-10 utamanya berfungsi sebagai penghambat produksi beberapa jenis sitokin (TNF, IL-1, kemokin, serta IL-12), serta menghambat fungsi makrofag serta sel dendritik untuk membantu aktivasi sel-T, sehingga bersifat imunosupresi. IL- 10 akan menekan ekspresi MHC-II pada * Departemen Ilmu Penyakit Mulut, Fakultas Kedokteran Gigi, Universitas Islam Sultan Agung ** Program Pendidikan Sarjana Kedokteran Gigi, Fakultas Kedokteran Gigi, Universitas Islam Sultan Agung *** Departemen Oral Biology, Fakultas Kedokteran Gigi, Universitas Islam Sultan Agung Correspondence : Rohman Mujayanto, Departemen Ilmu Penyakit Mulut Fakultas Kedokteran Gigi, Universitas Islam Sultan Agung, Jln. Raya Kaligawe KM. 4 Semarang 50012 ph. (024)6583584 fax. (024)6594366. Email : rochman.mujayanto@unissula.ac.id Keywords: Bay Leaf, IL-10, Traumatic Ulcers