Hindawi Publishing Corporation
Evidence-Based Complementary and Alternative Medicine
Volume 2013, Article ID 929074, 8 pages
http://dx.doi.org/10.1155/2013/929074
Research Article
Evaluation of Antidiabetic Activity and Associated Toxicity of
Artemisia afra Aqueous Extract in Wistar Rats
Taofik O. Sunmonu and Anthony J. Afolayan
Research Center for Phytomedicine, Department of Botany, University of Fort Hare, Alice 5700, South Africa
Correspondence should be addressed to Anthony J. Afolayan; aafolayan@uf.ac.za
Received 31 March 2013; Revised 15 May 2013; Accepted 20 May 2013
Academic Editor: Mohamed Eddouks
Copyright © 2013 T. O. Sunmonu and A. J. Afolayan. Tis is an open access article distributed under the Creative Commons
Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is
properly cited.
Artemisia afra Jacq. ex Willd. is a widely used medicinal plant in South Africa for the treatment of diabetes. Tis study aimed to
evaluate the hypoglycemic activity and possible toxicity efect of aqueous leaf extract of the herb administered at diferent dosages
for 15 days in streptozotocin-induced diabetic rats. Administration of the extract at 50, 100, and 200 mg/kg body weight signifcantly
( < 0.05) increased body weight, decreased blood glucose levels, increased glucose tolerance, and improved imbalance in lipid
metabolism in diabetic rats. Tese are indications of antidiabetic property of A. afra with 200 mg/kg body weight of the extract
showing the best hypoglycemic action by comparing favourably well with glibenclamide, a standard hypoglycemic drug. Te extract
at all dosages tested also restored liver function indices and haematological parameters to normal control levels in the diabetic rats,
whereas the kidney function indices were only normalized in the diabetic animals administered with 50 mg/kg body weight of the
extract. Tis investigation clearly showed that in addition to its hypoglycemic activity, A. afra may also protect the liver and blood
against impairment due to diabetes. However, some kidney functions may be compromised at high dosages of the extract.
1. Introduction
Diabetes mellitus is a major endocrine disorder and growing
health problem in most countries. It is a metabolic disease as
old as mankind; and its incidence is considered high all over
the world [1]. Increase in sedentary lifestyle, consumption
of energy-rich diets, and obesity are some of the factors
causing the rise in the number of diabetics. Te World
Health Organization (WHO) estimated diabetes in adults to
be around 173 million, and about two-thirds of these patients
live in developing countries [2]. Te prevalence of diabetes is
on the increase worldwide including South Africa; and it is
still expected to increase by 5.4% in 2025 [3]. WHO further
reports that over 4.8 million annual deaths (9% of global
total) are attributable to either diabetes or its complications
[4].
Despite these alarming statistics, there is no specifc
and defnite therapy currently for diabetes. However, a few
chemotherapeutic drugs have been in use to manage the
disease since the accidental discovery of the hypoglycemic
action of sulfonamides [5]. Te thrust of such management
measures is to achieve an efective blood glucose control or
utilization, with a view to delaying or averting the onset of
complications. Te application of these measures is, however,
limited due to their high cost and associated side efects.
Consequently, attention is being focused on the use of herbal
medicines for the treatment of diabetes.
In traditional African societies, phytotherapy is highly
valued and widely utilized. South Africa, particularly, has
remarkable biodiversity and rich cultural traditions of plant
use. Hence, it is not surprising why the majority of the
population in South Africa use plant materials as their source
of primary healthcare and as an alternative or supplement to
visiting western healthcare practitioners [6]. Tis is particu-
larly true for the treatment of diabetes, and WHO has also
authenticated phytotherapeutic approach to the treatment of
the disease [7, 8]. Te use of natural remedies for diabetes
treatment is also strengthened due to the belief that herbs can
provide some benefts over allopathic medicine and allows
users to feel that they have some control in their choice
of medication [9]. However, their general acceptability has
been limited by lack of dose regimen and adequate data