International Journal of Poultry Science 12 (8): 484-488, 2013 ISSN 1682-8356 © Asian Network for Scientific Information, 2013 Corresponding Author: Jein Rinny Leke, Faculty of Animal Husbandry, Brawijaya Uuniversity, Malang-65145, Indonesia 484 Egg Internal Quality and n-3 Fatty Acids of Native Chicken Fed on Skipjack Fish (Katsuwonus pelamis. l) Industrial Waste Containing Feed Jein Rinny Leke , Achmanu , Osfar Sjofjan and Marie Najoan 1,2 1 1 2 Faculty of Animal Husbandry, Brawijaya Uuniversity, Malang-65145, Indonesia 1 Faculty of Animal Husbandry, Sam Ratulangi University, Manado, North Sulawesi, Indonesia 2 Abstract: The objective of this study was to assess the effect of the use of skipjack fish industrial waste to native hen diets on egg internal quality. A total of 180 native laying hens 27-34 weeks of age were used in this experiment and randomly distributed into 180 units of battery cage. There were three types of skipjack waste used as main treatment diets, namely Heads and Entrails (HE) (S1), the rest of filleting (bones) (S2) and company sorting residuals (arachon) (S3). The treatment diets have five levels of skipjack waste each, hence the 15 treatment diets combination which consisted of one diet without skipjack waste (control) and the others four containing 5, 10, 15 and 20% skipjack wastes each. The experiment was carried out based on a nested completely randomized design, comprising three main treatments (A factors), five levels as sub- plot (B factors) with three replicates containing four native hens each. Data were analyzed using and the differences between treatments were tested Duncan's multiple range test. The variables measured were egg weight, Hen Day Production (HDP), egg weight, yolk color and n-3 FA. The results revealed that the main treatments HE, bones and arachon were not significantly effects (P>0.05) on HDP, egg weight and yolk color. Meanwhile, increasing the level of HE, Bones and Arachon did not affect significantly (P>0.05) against HDP, egg weight, yolk color, Furthermore, the treatments (HE, Bones, Arachon) were not significantly effects (P>0.05) on SAFA, MUFA, En-3, En-6, Ratio of n-3:n-6. Similarly, increasing the level of up to 20% of HE, Bones and Arachon to hen diets did not affect significantly (P>0.05) against SAFA, MUFA, E n-3, En-6 and ratio of n-3: n-6. Therefore, the skipjack industrial waste, as Head and Entrails (HE), bones and arachon could be used to the ration for optimizing productivity of native hen. It was recommended, the level of using the skipjack waste of up to 15% in ration. Key words: Internal quality, n-3 FA, skipjack fish industry waste INTRODUCTION Native chicken is one among animal resources which generally become a mainstay, as well as an economic buffer for the rural communities. This proved that the rural communities now prefer to keep native chicken, because it has special specifications on both the taste and aroma of its eggs and meat and the efficacy of native hen egg and meat can not be replaced by any of race chicken products. Various studies have been conducted on the characteristics and quality of chicken products especially egg and meat. Native hen eggs is one of livestock product which has a high nutritional value that can be accepted by all consumers. Public awareness of the native hen eggs which is a conventional food and medicine due to its bioactive components form of functional food which able to prevent the disease. This gets a particular interest in order to meet consumer demand and the quality of native chicken eggs, mainly on the content of amino acids and n-3 fatty acids. This can be done by feeding trials on chickens. Others function of omega-3 fatty acids (PUFA)-enriched eggs are decreases the risk of heart disease, inhibits the growth of prostate and breast cancer and is required for normal fetal brain and visual development (Lewis et al., 2000). Skipjack fish industrial waste has potential as an alternative feed source in Indonesia Skipjack industrial waste is a byproduct of making wooden fish and canning fish. North Sulawesi Utara is known as one of the region’s potential skipjack fishing ground in Indonesia. The production of skipjack in Sulawesi Utara in 2010 amounted to 60.168.1 tons/per year. PT Nichindo is one of the wooden fish export company in Sulawesi Utara. Skipjack waste is by-product from the process of making skipjack wooden fish, where one ton of wet skipjack waste can produce 250 kg of dry waste with 10 moisture content. Skipjack fish industrial waste is classified as Heads and Entrails (HE), the rest of filleting (Bones), company sorting residuals (Arachon). To get standardized as animal feed, it is necessary to test the materials trough laboratory proximate analysis and biological experiments, so it suitable to be used as poultry feed in improving product quality, both eggs and meat.