Jurnal Jamu Indonesia (2018) 3(2): 68-74 Artikel Penelitian Authors Beny Rahmanto 1* , Wawan Halwany 1 , Fajar Lestari 1 , Khoerul Anwar 2 , Liling Triyasmono 2 , Muhammad Ikhwan Rizki 2 , Maman Turjaman 3 Affiliation 1 Environtment and Forestry Research and Development Institute of Banjarbaru, South Kalimantan, Indonesia 2 Department of Pharmacy, Faculty of Mathematic and Natural Sciences, Universitas Lambung Mangkurat, South Kalimantan, Indonesia 3 Forest Research and Development Center, Bogor, West Java, Indonesia Keyword  Aquilaria microcarpa Baill leaf  Extract standardization  Non-specific parameter  Specific parameter Received 3 May 2018 Revised 16 June 2018 Accepted 6 August 2018 * Corresponding author Beny Rahmanto Jl. A. Yani Km 28,7 Banjarbaru, Kalimantan Selatan, Indonesia 7072 ben_rahmanto@yahoo.com ABSTRACT The aim of this research is to determine the specific and non-specific parameter from ethanolic extract of A. microcarpa. Specific parameters such as the organoleptic properties, content of water and ethanol soluble compound, and chhromatogram profile of the extract. In contrast, the non-specific parameter includes drying losses, ash levels, microbiological contamination levels, and heavy metal contamination levels. The results showed that the organoleptic properties of ethanolic extract of A. microcarpa was thick, blackish brown color, bitter taste and have distinctive odor. The average content of water-soluble compounds of 66.93%, ethanol-soluble of 47.97%, and chromatogram profile with Rf 0.636. The non-specific parameters results were drying losses of 5.50%, total ash content of 3.73%, acid-insoluble ash of 2.13%, microbial contamination testing results of 1.2x102-5.5x103 colonies/g, and total mold/ yeast contaminants of 10-100 colonies/g. Heavy metal contamination Pb levels and Cd levels were 5.47 mg/kg and 0.19 mg/kg respectively. These studies suggested that the observed specific and non-specific parameters may be helpful for establishing pharmacopoeia standards of A. microcarpa and to ensure uniformity of extract effect. INTRODUCTION Agarwood is one of non-timber forest products (NTFPs) which has high economic value. Agarwood-producing plants are widely known as members of the Thymelaeaceae family, Aquilaria and Gyrinops genus. They can grow in the temperature range of 24-32 o C, humidity of 80-90%, with rainfall of 1,000-1,500 mm/year (Jensen and Meilby 2008). The land condition for growing of agarwood producing trees are mostly classified as podsolic soil with sandy clay or marginal land with altitude of 10-400 m a.s.l. One type of them have been widely known cultivated by agarwood producers is Aquilaria microcarpa (Paoli et al. 2001). Agarwood has many benefits such as anti-asthmatic, stomach pain medication, hepatitis, and cirrhosis (Saidu et al. 2014; Fatmawati and Hidayat 2016). Ethanol extract Characterization of Ethanol Extract from Agarwood ( Aquilaria microcarpa Baill.) Leaf