_____________________________________________________________________________________________________ *Corresponding author: E-mail: amalarmathi@gmail.com; Annual Research & Review in Biology 35(3): 1-13, 2020; Article no.ARRB.54704 ISSN: 2347-565X, NLM ID: 101632869 A Comparative Study of in vivo Plant and in vitro Callus Extracts of Centratherum punctatum Cass K. Mercy Madhumitha 1 , J. Anbumalarmathi 1* , S. Aruna Sharmili 1 , G. Nandhini 1 and G. Shanmuga Priya 1 1 Department of Biotechnology, Stella Maris College (Autonomous), Chennai, 600086, India. Authors’ contributions This work was carried out in collaboration among all authors. Author KMM performed the statistical analysis and wrote the protocol. Author JA designed the study and managed the literature search and wrote the first draft of the manuscript. Authors JA and SAS managed the analyses of the study. Authors GN and GSP performed the statistical analyses. All authors read and approved the final manuscript. Article Information DOI: 10.9734/ARRB/2020/v35i330195 Editor(s): (1) Dr. Bechan Sharma, University of Allahabad, India. Reviewers: (1) Aba-Toumnou Lucie, University of Bangui, Central African Republic. (2) Esmat Anwar Abou Arab, National Research Center, Egypt. Complete Peer review History: http://www.sdiarticle4.com/review-history/54704 Received 05 January 2020 Accepted 10 March 2020 Published 18 April 2020 ABSTRACT The present study aims at comparative study between plant and callus extract, with respect to analysis of phytochemical constituents, antioxidant, antibacterial activity and cytotoxicity properties of Centratherum punctatum using aqueous medium and different solvents such as, methanol and ethyl acetate. In vitro studies in MS media supplemented with BAP 4.5 mg/L + Kn 4.0 mg/L has shown the high callus induction percentage of 92.33% with a maximum callus weight of 1.08 g. The phytochemical analysis of aqueous, methanol and ethyl acetate extract of C. punctatum in vivo plant and in vitro callus showed the presence of alkaloids, flavonoids, phenols and carbohydrates. The aqueous extract of both plant and callus showed the presence of tannins, proteins and steroids whereas the methanol extract showed the presence of tannins, amino acids and terpenoids. The ethyl acetate extract showed terpenoids and protein. FTIR analysis of plant and callus aqueous extract had a maximum characteristic band at 3399.87 cm -1 and 3412.73 cm -1 respectively indicating the presence of N-H stretching. GC-MS analysis revealed the presence of 11 different compounds in ethyl acetate extracts of plant and the callus extract revealed the presence of 15 different compounds which was absent in the plant extract. Plant extract exhibited maximum total phenol content than callus extract. The in vitro callus extract showed higher DPPH radical scavenging Original Research Article