Jentashapir J Health Res. 2014 August; 5(4): e21881. DOI: 10.5812/jjhr.21881 Published online 2014 August 11. Letter Extracellular Esterase Secretion by Vaginal Isolates of Candida Albicans Zahra Seifi 1 ; Ali Zarei Mahmoudabadi 1,2,* 1 Department of Medical Mycology, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IR Iran 2 Health and Research Institute, Infectious and Tropical Diseases Research Centre, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IR Iran *Corresponding author: Ali Zarei Mahmoudabadi, Department of Medical Mycology, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IR Iran. Tel: +98- 6113330074, Fax: +98-6113332036, E-mail: zarei40@hotmail.com Received: October 5, 2013; Revised: October 7, 2013; Accepted: October 30, 2013 Keywords:Esterase; vagina; Candida albicans Copyright © 2014, Ahvaz Jundishapur University of Medical Sciences; Published by Kowsar. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited. Many of the pathogenic Candida species have the ability to secrete extracellular lipolytic enzymes such as esterases and phospholipases (1, 2). These enzymes are categorized into two important types: proteinases, which hydrolyze peptide bonds; and lipolytic enzymes (phospholipase and esterases), which hydrolyze lipid compounds in host cells tissues (3). Destroying or deranging constituents of host cell membranes due to hydrolytic enzymes causes dysfunction and/or cells disruption. Adherence and tis- sue penetration by Candida species will be facilitated by secreted extracellular enzymes and therefore, host inva- sion occurs (4, 5). Khosravi et al. examined esterase activ- ity of Candida albicans as a potential virulence factor in animal model (5). The esterase activities of these yeasts were previously demonstrated with the application of the tween opacity test medium with different tween com- pounds (2) and colorimetery method (6). The aim of the present study was to investigate esterase activities on vaginal isolates of C. albicans. A total of 85 vaginal isolates of C. albicans were used. The identities of the isolates were confirmed according to their morphol- ogy on CHROMagar Candida (CHROMagar Candida, Paris, France), cornmeal agar (HiMedia, India), and germ tube production (7). Esterase activity was determined by visual methods presented by Slifkin (8). The agar medium con- sisted of 1 g of peptone (Difco, USA), 0.5 g of NaCl (Mer- ck, Germany), 0.01 g of CaCl 2 (Merck, Germany), and 1.5 g of agar (Merck, Germany). Medium was sterilized and cooled to about 50℃; then 0.5 mL of sterile tween 80 (Merck, Germany) was added. An overnight culture of each isolate was inoculated into medium by touching the center of the agar medium with a cotton swab in triplicate. The inoculated culture plates were incubated at 29℃ and were examined during ten days. The presence of a halo around an inoculated site was indicative of a positive test and a sign of esterase pro- duction by the Candida isolate. Esterase activity (Ez value) was reported as follows: a Ez of 1.0 as negative (-), 0.90 to 0.99 as weak (+), 0.80 to 0.89 as mild (+ +), 0.70 to 0.79 as strong (+ + +), and < 0.69 as very strong (+ + + +) (2). The results were calculated from triplicate tests for each isolate. In the present study, 23.5% of isolates showed es- terase activity after two days, 47.1% after three days, 14.1% after four days, 10.6% after five days, and 4.7% after seven days. The highest esterase activity (Ez value < 0.40) was observed in 21.2% of isolates (Table 1). Aktas et al. (9) have shown that 98.3% of C. albicans strains yielded positive results for esterase activity in two to ten days. In addition, all tested isolates of C. albicans and C. Candida tropicalis showed esterase activity during two to three days by Slifkin (8). All of the Aktas et al. (9) tested C. tropicalis and C. Candida guilliermondii strains had positive results for esterase activity whereas most of the tested C. Candida parapsilosis and C. Candida metapsilosis by Ge et al. (2) failed to show esterase activity. Pakshir et al. tested C. albicans from onychomycosis and oral lichen planus patients for esterase activity (10). Most tested spe- cies produced esterase enzyme during three days and no significant difference was seen between the two groups of C. albicans. In the presents study, all C. albicans strains were isolated from patients with vulvovaginal candidia- sis and were definitely considered as pathogen with Ez ≤ 60 in all strains; however, there was a difference among Candida strains with regard to the production of esterase (Table 1). Ge et al. in their study found Ez < 0.69 for es- terase activity among tested Candida species, which was determined as 4 + (2). In conclusion, our results showed that all isolates yielded positive results; however, variable levels of esterase activity were observed during incuba- tion period.