- S1 - Electronic Supplementary Information (ESI) Enhancement of the photocytotoxic efficiency of sub-12-nm therapeutic polymeric micelles with increased co-localisation in mitochondria Kyuha Chong a , Taeyun Ku b , Phei Er Saw c , Sangyong Jon c , Ji-Ho Park *b , Chulhee Choi *a,b a Graduate School of Medical Science and Engineering, KAIST, 291 Daehak-ro, Yuseong-gu, Daejeon 305-701, Republic of Korea. b Department of Bio and Brain Engineering, KAIST, 291 Daehak-ro, Yuseong-gu, Daejeon 305-701, Republic of Korea. Fax: +82-42-350-4380; Tel: +82-42-350-4361; E-mail: jihopark@kaist.ac.kr or cchoi@kaist.ac.kr c Department of Biological Science, KAIST, 291 Daehak-ro, Yuseong-gu, Daejeon 305-701, Republic of Korea. * Correspondence to: Ji-Ho Park and Chulhee Choi Table of Contents Experimental Section S2-8 1. Materials 2. Preparation of the drug delivery systems (DDSs) 3. Characterisation of the polymeric micelles 4. Cell cultivation 5. Determination of dark cytotoxicity and cellular uptake 6. Determination of the DDS-dependent photocytotoxic efficiency and intracellular co- localisation of HY 7. Image processing and statistical analysis Supplementary Figures and Tables S9-14 References S15 Electronic Supplementary Material (ESI) for Chemical Communications This journal is © The Royal Society of Chemistry 2013