Open Access Research Article Open Access Saei et al., J Aquac Res Development 2017, S2 DOI: 10.4172/2155-9546.S2-013 Research Article Open Access Journal of Aquaculture Research & Development J o u r n a l o f A q u a c u lt u r e R e s e a r c h & D e v e l o p m e n t ISSN: 2155-9546 ISSN: 2155-9546 JARD, an open access journal J Aquac Res Development Current and Emerging Diseases/Disorders of Fish in Aquaculture Keywords: Biotox; Growth performance; Enzymatic activity; Rainbow trout; Afatoxin Introduction Afatoxins are produced by Aspergillus fungi, which can infect many potential feedstufs as corn, peanuts, rice, fsh meal, shrimp and meat meals [1]. Te afatoxin B1 (AFB1) is very toxic and exists in a wide range of mycotoxins. Its high toxicity to both animals and humans makes AFB1 the most dangerous mycotoxin [2]. Liver is the target organ of afatoxin B1, long-term intake of diets containing AFB1 can cause a reduction of weight gain, growth stunting and immunosuppression in animals, while increasing hepatocellular carcinoma incidence [3]. It seems that efects of afatoxins on fsh depend on the fsh species and age, as fsh fry are more susceptible than adults and species like Rainbow trout, (Onchorhynchus mykiss) and European seabass, (Dicentrarchus labrax) seem to be more susceptible than channel catfsh, (Ictalurus punctatus) [4-6]. Tese species specifc diferences can be attributed to diferences in the metabolism of AFB1 in the liver and afnity of AFB1-derived metabolites to hepatic macromolecules [7]. Te negative efcts of afatoxin in diferent species such as Rainbow trout, Baltic salmon (Salmo salar), Nil tilapia (Oreochromis niloticus), coho salmon (Oncorhynchus kisutch), and Red Drum (Sciaenops ocellatus) [4,8-11] and shrimp (Litopenaeus vannamei) have been demonstrated. Tere are a number of approaches that can be taken to protect of animal from toxic efect of afatoxins and these involve prevention of fungal growth, and strategies to reduce or eliminate mycotoxins from contaminated feeds, such as physical, chemical, nutritional, and biological techniques [12]. In the feed industry, the most common method to control AFB1 is the application of nonnutritive adsorptive materials so as to reduce the concentration of fungal toxins in the gastrointestinal tract of animals [13] . Te aim of these additives is to inhibit the uptake of mycotoxins by an animal in vivo. Te use of mycotoxin binding agents is occasionally recommended to farmers in order to protect animals against the harmful efects of mycotoxins occurring in contaminated feeds. Te efcacy of the adsorption appears to depend on the chemical structure of both the adsorbent and the mycotoxin [14,15]. Most studies related to the alleviation of mycotoxicosis by the use of adsorbents are focused on aluminosilicates, mainly zeolites, and aluminosilicate-containing clays, all consisting of aluminates, silicates and some interchangeable ions, mainly alkali metal and alkaline earth metal ions [14,16,17]. Mineral clay products such as bentonites, zeolites, and aluminosilicates are the most common feed additives which are efective in binding/ adsorbing afatoxins [15]. In the other hand, yeast or yeast cell walls show a potential as mycotoxin binders Besides its excellent nutritional value [14]. Biotox is one of the mycotoxin adsorbing agent and consists of various silicates combined with extracts of the cell walls of a yeast (Saccharomyces cerevisiae) that we have used in our study. Te number of studies addressing the efects of the addition toxin binders to fsh feed is very limited. More research is needed to better understand the potential efects of these compounds in diet. Terefore, in the present study attempted was conducted to examine the infuence of afatoxin on growth performance, survival, enzymatic activity, hematological and biochemical parameters of fngerling rainbow trout (Oncorhynchus mykiss) and the antitoxic efect of Biotox. *Corresponding author: Mohsen Mohamadi Saei, Lorestan Agricultural and Natural Resources Research and Education Center, AREEO, Khorramabad, Iran, Tel: +98 17 3222 0321; E-mail: mohsenmohamadi57@gmail.com Received December 23, 2016; Accepted January 19, 2017; Published January 21, 2017 Citation: Saei MM, Taee HM, Siahpoust S, Taheri M (2017) Effects of Toxin Binder Biotox on Growth Performance Survival, Enzymatic Activity, Hematologichal and Biochemical Parameters of Fingerlings Rainbow Trout (Oncorhynchus mykiss) Fed Diets-Contaminated with Afatoxin. J Aquac Res Development S2: 013. doi:10.4172/2155-9546.S2-013 Copyright: © 2017 Saei MM, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Abstract The aim of the study was to determine the effect of Biotox on growth performance, survival, enzymatic activity, hematological and biochemical parameters of fngerlings rainbow trout fed with afatoxin. For this purpose, 900 fsh were stocked in four treatments at the pools and fed 4 times a day by supplementing the basal diet with 0.1% Biotoxin +1 mg/kg afatoxin (T1), 0.2% Biotoxin +1 mg/kg afatoxin (T2), 1 mg/kg afatoxin (T3) and basal diet as control group (T4) for 60 days. There were signifcant differences (P<0.05) in survival in fsh fed different diets for 60 days. Survival was higher in the T2. there were no signifcant difference (P>0.05) was observed in growth performance, activity Alkaline Phosphatase (ALP) and hematological parameters, of fsh in different treatments. Also, no signifcant difference (P> 0.05) in serum globulin, albumin and total protein was observed between treatments and control group. The results of the present study showed a signifcant (P<0.05) increase in Glucose and triglyceride in T3, whereas serum cholesterol was higher in T3 and control group. Addition of 1-2 g Biotox/ kg feed did not affected growth performance and hematological parameters, but increased survival of fngerling fed with the diets of afatoxin B1-exposed. Effects of Toxin Binder Biotox on Growth Performance Survival, Enzymatic Activity, Hematologichal and Biochemical Parameters of Fingerlings Rainbow Trout (Oncorhynchus mykiss) Fed Diets- Contaminated with Aflatoxin Mohsen Mohamadi Saei 1 *, Hadis Mansouri Taee 2 , Sokaine Siahpoust 3 and Majid Taheri 3 1 Lorestan Agricultural and Natural Resources Research and Education Center, AREEO, Khorramabad, Iran 2 Fisheries Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran 3 Research and Development Expert Division, Arya Dalman. Co, Tehran, Iran