214 Padjadjaran Journal of Dentistry 2018;30(3):214-219. # Corresponding author: Janti Sudiono, Department of Oral Pathology, Faculty of Dentistry, Trisakti University, Indonesia. Jl. Kyai Tapa 1, West Jakarta, DKI Jakarta, 11440. Phone: 628159112336; Email: jantish@hotmail.com P-ISSN 1979-0201, e-ISSN 2549-6212 Available from:http://jurnal.unpad.ac.id/pjd/article/view/16851 DOI:10.24198/pjd.vol29no3.16851 Submission: May 18, 2018; Accepted: Oct 15, 2018; Publishing online: Nov 30, 2018 Macrophage and angiogenesis intensity within proliferative non-neoplastic and neoplastic oral lesions in-accordance with biological properties Janti Sudiono *# , Barnabas Howuk * , Cindy Fransisca * * Department of Oral Pathology, Faculty of Dentistry, Trisakti University ABSTRACT Introduction: The most common chronic infammation involved dental and oral tissue are gingival polyp, pulp polyp, and fbroma, that are proliferative non-neoplastic and neoplastic condition. The previous study revealed that increasing macrophage followed by increasing angiogenesis intensity. Increasing angiogenesis or vascular proliferation indicates progressive growth in the form of proliferative non- neoplastic or neoplastic disease outside of easily bleeding clinical features. This study was aimed to evaluate the macrophage and angiogenesis intensity within lesions, and the conformity with their biological properties. Methods: Samples used were taken from oral mucosa excision with clinical diagnose of gingival polyp (n = 3); pulp polyp (n = 3); and fbroma (n = 3). The macrophage was detected using immunostaining with CD68 antibody, resulted in brown staining of cell membrane under light- microscope; while angiogenesis intensity was evaluated as numbers of blood vessels HE staining. Result: The CD68 + expression as macrophage marker showed no signifcant diferences (p = 0.102 > 0.05) with the highest found in fbroma (51.32 ± 31.64%), followed by non-neoplastic pulp polyp (45.82 ± 15.94%), and the least in gingival polyp (29.98 ± 13.51%). One way-ANOVA test showed signifcant diferences (p = 0.02 < 0.05) in angiogenesis intensity, with the highest found in pulp polyp (12.00), followed by fbroma (11.81), and gingival polyp (9.67). However, there was no signifcant diference in angiogenesis intensity between non-neoplastic lesion (pulp polyp) and neoplastic lesion (fbroma) with p = 0.102 > 0.05. The Pearson R-test showed a mild positive correlation of angiogenesis intensity and CD68 + as a macrophage marker (r = 0.31). Conclusion: Macrophage and angiogenesis intensity, and their correlation within oral proliferative non-neoplastic and proliferative neoplastic lesions were in accordance with their biological properties. Keywords: Angiogenesis, macrophage, CD68 + , oral proliferative non-neoplastic and neoplastic lesion