International Journal of Research in Dermatology | May-June 2020 | Vol 6 | Issue 3 Page 341 International Journal of Research in Dermatology Sriarumtias FF et al. Int J Res Dermatol. 2020 May;6(3):341-346 http://www.ijord.com Original Research Article Splash mask formulation of tangerine (Citrus reticulata Blanco.) peel extract and turmeric (Curcuma longa L) extract as a whitening agent Framesti Frisma Sriarumtias*, Nurul Auliasari INTRODUCTION Hyperpigmentation is a skin problem caused by an increase in skin pigment. Increased melanin synthesis locally or uneven distribution of melanin which can cause local pigmentation or dark spots. 1-3 Melanin in the skin serves to absorb exposure to ultraviolet light, bind to free radicals produced in the cytoplasm, and protect the body from radiation exposure. Melanin as one of the dominant pigment components of skin color, plays a role in determining skin color variations between individuals. Melanin synthesis process occurs in melanosomes that are inside melanocytes. Melanosomes then move towards keratinocytes through dendrites. In keratinocytes, melanosomes release melanin, which is then distributed in epidermal keratinocytes. 1 In addition to turmeric, tangerines are widely used by people as a whitener because they contain large amounts of vitamin C, where vitamin C is a whitening agent with a mechanism of action that is exfoliation. 4-8 It is hoped that the combination of these two products can increase the effectiveness in inhibiting the tyrosinase enzyme which plays a role in melanin synthesis. In addition, the Splash Mask formulation is expected to be able to maximize this effect, because the Splash Mask formulation is made with a concentrate system, where both ingredients are added to the solvent until the condition is saturated. With the high concentration of the splash mask, how to use it by diluting it in water. 9 Basically, splash mask is a form of mask in the form of liquid with high concentration and is intended for people ABSTRACT Background: Indonesia is a tropical country that is exposed to the sun for 12 hours, one of the harm effects of sunlight which is damage to the skin. The purpose of this study was to determine tyrosinase inhibition of tangerin peel extract (Citrus reticulata Blanco.) from Garut, West Java, Indonesia then develop the splash mask from the extract. Methods: The tangerine peel will be extracted by maceration method. The splash mask formulation containing tangerine peel extract which has a whitening agent. Tyrosinase inhibition determine by ELISA method with tyrosinase enzyme and L-DOPA substrat. Results: The results showed that tangerine peel extract had tyrosinase inhibition with the IC50 of 30.000 ppm, which has less effective than kojic acid with IC50 81,10 ppm. Splash mask containing of 1000x IC50, evaluation carried out for 28 days showed that preparations made pharmaceutically stable. Conclusions: In addition, the whitening agent of tangerine peel extract is in the weak category. It is hoped that this research can increase the value of tangerine peel waste. Keywords: Splash mask, Tangerines peel extract, Tyrosinase, Whitening agent Department Pharmacy, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Garut, West Jawa Barat, Indonesia Received: 29 November 2019 Revised: 13 February 2020 Accepted: 02 March 2020 *Correspondence: Framesti Frisma Sriarumtias, E-mail: framesti@uniga.ac.id Copyright: © the author(s), publisher and licensee Medip Academy. This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial License, which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. DOI: http://dx.doi.org/10.18203/issn.2455-4529.IntJResDermatol20201577