_____________________________________________________________________________________________________ *Corresponding author: E-mail: krjadhav25@gmail.com; Journal of Pharmaceutical Research International 33(44A): 292-307, 2021; Article no.JPRI.74290 ISSN: 2456-9119 (Past name: British Journal of Pharmaceutical Research, Past ISSN: 2231-2919, NLM ID: 101631759) Formulation and Evaluation of Miconazole Nitrate Loaded Novel Nanoparticle Gel for Topical Delivery Khanderao Jadhav 1* , Shivraj Jadhav 1 , Deepak Sonawane 1 , Deepak Somvanshi 1 , Hina Shah 1 and Pratik Patil 2 1 Department of Pharmaceutics, Shreeshakti Shaikshanik Sanstha’s Divine College of Pharmacy, Satana, 423301, Maharashtra, India. 2 KBHSSs Trusts Institute of Pharmacy College, Malegaon, 423105, Maharashtra, India. Authors’ contributions This work was carried out in collaboration among all authors. All authors read and approved the final manuscript. Article Information DOI: 10.9734/JPRI/2021/v33i44A32616 Editor(s): (1) Dr. Paola Angelini, University of Perugia, Italy. (2) Dr. Takashi Ikeno, National Institute of Mental Health, National Center of Neurology and Psychiatry, Japan. Reviewers: (1) Mahdi Jufri, Universitas Indonesia, Indonesia. (2) Aymn Yaseen Sharaf Zeebaree, Duhok Polytechnic University, Iraq. Complete Peer review History: https://www.sdiarticle4.com/review-history/74290 Received 06 July 2021 Accepted 16 September 2021 Published 18 September 2021 ABSTRACT The aim of the present research work is to design miconazole-loaded chitosan nanoparticles that could potentially assemble in wrinkle and hair follicles to provide prolong release to the skin tissue. The amount of drugs required for the preparation of nanoparticles was determined by studying the entrapment efficiency of preliminary batches. The emulsification/Solvent evaporation method was used for the preparation of nanoparticles. Different proportions of Miconazole Nitrate and Chitosan were dissolved in DCM. The size of the globules in the emulsion was reduced by a high energy shearing using a probe Sonicator at 50 % amplitude for 10 Minutes, followed by the addition of 10 ml 2% PVA. After overnight evaporation of DCM, for isolation of dried NPs, the NPs dispersion was centrifuged at 15,000 RCF for 30 minutes. The obtained particles were dispersed in de-ionized water and freeze-dried. 3 2 full factorial design was selected for optimization purposes. Prepared batches were evaluated for various parameters such as entrapment efficiency, production yield, particle size, zeta potential, and SEM. F5 batch was found to be optimized which was then used for the preparation of gel. Three levels of Carbopol934 and propylene glycol were used for the Original Research Article