Health, 2017, 9, 252-260 http://www.scirp.org/journal/health ISSN Online: 1949-5005 ISSN Print: 1949-4998 DOI: 10.4236/health.2017.92017 February 15, 2017 Association between Serum Glutathione Peroxidases and Superoxide Dismutases mRNA Level with Coronary Artery Disease Ali Reza Abaspour 1 , Mohammad Taghikhani 2 , Mohamad Reza Parizade 3 , Mohsen Moohebati 4 , Fahime Ghafoori 1 , Mehraneh Mehramiz 3 , Maryam Tayefi 5 , Amir Avan 5 , Marzeye Ghalandari 6 , Gordon A. A. Ferns 7 , Majid Ghayour-Mobarhan 3,4,5* 1 Department of Molecular Science, Faculty of Medicine, North Khorasan University of Medical Science, Bojnurd, Iran 2 Department of Clinical Biochemistry, Tarbiat Modares University, Tehran, Iran 3 Department of Biochemistry and Nutrition, Faculty of Medicine, Mashhad University of Medical Science (MUMS), Mashhad, Iran 4 Cardiovascular Research Center, Avicenna Research Institute, Mashhad University of Medical Science (MUMS), Mashhad, Iran 5 Department of Modern Sciences and Technologies, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran 6 Department of Biochemistry, Faculty of Basic Science, Payame Noor University, Mashhad, Iran 7 Division of Medical Education, Brighton & Sussex Medical School, Brighton, UK Abstract Background: Oxidative stress plays a crucial role in the pathogenesis and progression of many diseases, including cardiovascular disease (CVD) and di- abetes mellitus. Oxidative stress results from an imbalance between free radi- cal formation and the protective antioxidant mechanisms. The latter mechan- isms include superoxide dismutases (SODs) and glutathione peroxidases (GPx) that scavenge excessive ROS and protect cells against excess ROS production. The aim of current study was to determine the serum levels of SOD and se- rum GPx mRNA as well as the serum prooxidant-antioxidant balance in CVD patients. Method: A total of 103 subjects were recruited, with ≥50% stenosis (Angio + ) or <50% stenosis of one or more coronary arteries by angiography (Angio − ). The expression levels of SOD and GPx in serum were measured us- ing real time PCR. Biochemical-analyses (e.g., triglycerides; high-density lipo- protein cholesterol; low-density lipoprotein cholesterol; fasting-blood-glucose) were determined in all the subjects. Associations of SOD and GPx levels with biochemical and anthropometric characteristics were assessed together with evaluation of the serum pro-oxidant-antioxidant balance (PAB). Results: CVD subjects had a significantly higher level of fasting blood glucose (FBG), TC, LDL-C, TG and hs-CRP levels, as compared to control subjects. The level of serum PAB was significantly higher in the CVD group, 117.92 ± 35.51 and 110.65 ± 27.65 µg/dl in the angio − and angio + groups, respectively compared to How to cite this paper: Abaspour, A.R., Taghikhani, M., Parizade, M.R., Mooheba- ti, M., Ghafoori, F., Mehramiz, M., Tayefi, M., Avan, A., Ghalandari, M., Ferns, G.A.A. and Ghayour-Mobarhan, M. (2017) Asso- ciation between Serum Glutathione Perox- idases and Superoxide Dismutases mRNA Level with Coronary Artery Disease. Health, 9, 252-260. https://doi.org/10.4236/health.2017.92017 Received: May 20, 2016 Accepted: February 12, 2017 Published: February 15, 2017 Copyright © 2017 by authors and Scientific Research Publishing Inc. This work is licensed under the Creative Commons Attribution International License (CC BY 4.0). http://creativecommons.org/licenses/by/4.0/ Open Access