Influence of stratification duration and hormone concentration in aonla (Emblica officinalis, GAERTN.) Surya Narayan Department of Horticulture, K.A.P.G., Collage Allahabad, 211001, (India) Email: sunara.kapg@gmail.com Abstract The experiment was conducted at the Department of Horticulture, Kulbhasker Ashram Post Graduate Collage, Allahabad, Uttar Pradesh with a view to standardize suitable stratification duration and hormone concentration for Aonla seed treatment. There were ten treatment combinations (T 1 to T 10 ) including a control. Different duration of seed stratification i.e., 24hours,48 hours and 72hours were tried along with the 100ppm,200ppm and 300 ppm GA 3 seed treatment . Treated seeds were sown in the polythene bags (25x15 cm size, 200gauge thick) containing soil, sand and FYM mixture (1:1:1). It was interesting to note that the effect of stratification duration and hormone treatment concentration was found to be significant for seed germination, transplanting success, seedling mortality percentage and rate of seed germination. Treatment T 6 (48hrs+300ppmGA3) yielded highest percentage, (84.00) of seed germination while the lowest percentage value (37.25) was recorded in T 9 (72hrs+300ppmGA3) treatment and the transplanting success was also lowest in T 9 . The seedling mortality percentage was maximum (79.25) with T 9 where as lowest percentage value (22.00) was observed for T 6 treatment. It may be concluded that T 6 treatment can be recommended for the better stand establishment of Aonla nursery. Key Words: Stratification, Treatment, GA 3, Nursery, Aonla, Mortality, Seedling, Germination, Seed. Paper Cited: Narayan, S. (2017). Influence of stratification duration and hormone concentration in aonla (Emblica officinalis, GAERTN.). South Asian Journal of Food Technology and Environment, 3(2): 581-584. Introduction Increased demand of aonla (Emblica officinalis, Garten.) buddlings in traditional as well as nontraditional areas of India due to its peculiar character of diverse use, medicinal value, tolerance to biotic and abiotic stresses, higher benefit cost ratio and positive government policies emphasized to chalk out some feasible and acceptable measures for the better stand-establishment of saplings at the nursery stage. Aonla buddlings are prepared thorough budding on seedling root - stock which is obtained through seeds. In nature, aonla seed has poor germination and higher seedling mortality, owing to adverse edaphic conditions during nursery stage. Therefore it becomes imperative to standardize suitable stratification time and exact hormone concentration for seed treatment for flourishing the aonla nursery-industry. Certainly, these tactics are the most important component to provide sound base for propagation, once time and concentration is standardize, we shall be able to grow healthy seedlings with faster rate. Keeping these aspects in view, the experiment was under taken to ascertain the effect of the stratification and hormone treatment on seed germination, rate of seed germination, transplanting success and mortality of seedlings. Materials and Method The experiment was conducted at the Department of Horticulture, Kulbhasker Ashram Post Graduate Collage, Allahabad, Uttar Pradesh during the year 2012-13 with a view to standardize suitable stratification duration and hormone concentration for Aonla seed treatment. There were ten treatment combinations (T 1 to T 10 ) including a control. Different duration of seed stratification i.e., 24hours, 48 hours and 72hours were tried. Soaked seed were put in layers under different strata of moist sand for varying duration. GA 3 hormone @ 100ppm, 200ppm and 300 ppm was used for seed treatment after stratification. Treated seeds were sown in the polythene bags (25x15 cm South Asian J. Food Technol. Environ., 3(2): 581-584 (2017) ISSN 2394-5168(Print), 2454- 6445(online) www.sweft.in South Asian J. Food Technol. Environ., 3(2):581-584 (2017) [581]