27th World Congress on Ultrasound in Obstetrics and Gynecology Poster discussion hub abstracts Objectives: To determine correlation between macroscopic appear- ances of superficial endometriotic lesions noted at laparoscopy and the final histopathology diagnosis. Methods: A prospective cohort study involving consecutive women with chronic pelvic pain who underwent laparoscopy between January 2015 - December 2016. The laparoscopic findings were recorded in terms of location, number and morphological description (i.e. red spot, white spot, pocket and/or fibrous adhesion) of the endometriotic lesions. These findings were correlated with the final histopathological diagnosis. All endometriotic lesions were excised at the time of laparoscopy. Results: A total of 77 consecutive women underwent laparoscopy. 9/77 were noted to have deep infiltrating endometriosis with bowel involvement and were excluded. 4/77 had a negative laparoscopy with normal pelvis. 64/77 had visualised lesions suspicious of superficial endometriosis. The mean age of the women was 27.7±7.7 y, 37% were nulliparous, 27.5% had previous history of endometriosis. A total of 180 biopsies were collected .The description of lesion were: red spots74/180, white spot 87/180, peritoneal pocket 11/180 and adhesions 8/180. Overall histopathological confirmation rate for endometriosis was 56%.There was no correlation between lesion distribution, morphology and positive diagnosis of endometriosis (p-value=0.8). These parameters were not statistically different in the presence of past history of endometriosis (p-value=0.19). Conclusions: Although the numbers in this study are small, there is no correlation between the macroscopic appearance of superficial endometriosis lesions and gold standard histological confirmation. P19: FETAL ECHOCARDIOGRAPHY II P19.01 Application of different frame rates in two-dimensional speckle-tracking in fetal echocardiography and their influence on strain analysis C. Enzensberger 1 , L. Rostock 1 , F. Achterberg 1 , O. Graupner 2 , A. Wolter 1 , J. Herrmann 3 , R. Axt-Fliedner 1 1 University Hospital UKGM, Justus-Liebig University, Department of Obstetrics and Gynecology, Division of Prenatal Medicine, Giessen, Germany; 2 Department of Obstetrics and Gynecology, Klinikum Rechts der Isar, Technical University of Munich, Munich, Germany; 3 Statistical Consulting Service, Giessen, Germany Objectives: Frame rates (FR) used for strain analysis assessed by speckle tracking in fetal echocardiography show a huge variation. Due to high fetal heart rates, appropriate high FR are requested for application in the fetus. The aim of this study was to investigate the influence of the FR on strain analysis in two-dimensional (2D) speckle tracking. Methods: A prospective cross-sectional study was performed. Based on an apical or basal four-chamber view of the fetal heart, cine loops were acquired on a Toshiba Aplio 500 system. Each loop was digitally stored twice: firstly as a DICOM (digital imaging and communications in medicine) file with a FR of 60 frames per second (fps), secondly with the original FR (acoustic FR = AFR). For each loop, fetal global longitudinal peak systolic strain (LPSS) values of both, left (LV) and right ventricle (RV), were assessed by 2D Wall Motion tracking. Results: A total of n=48 healthy fetuses were included. Mean gestational age was 26.5 ± 5.5 weeks. The AFR was 129 ± 23 fps. Relating to global LPSS values of both ventricles, there was a significant difference between DICOM FR and AFR (LV: -17.3% ± 3.6% (DICOM FR) vs. -15.0% ± 3.1% (AFR); RV: -15.9% ± 3.2% (DICOM FR) vs. -14.0% ± 3.1% (AFR); p<0.001). Conclusions: Fetal LPSS values of both, LV and RV, assessed with high AFR, were significantly lower than the corresponding LPSS value analysed with DICOM 60 fps. Ongoing studies need to clarify the correlation of LPSS values acquired with different FR. P19.02 The effects of maternal diabetes on fetal heart function: a systematic review A.L. Depla, L. de Wit, R. de Heus, B.B. van Rijn, M.N. Bekker University Medical Centre Utrecht, Utrecht, Netherlands Objectives: The incidence of diabetes in pregnancy is rising. Growing evidence shows not only an association with structural anomalies of the fetal heart, but also functional changes. This review aims to give an overview of the possible effects of diabetes on fetal cardiac function. Methods: Databases were searched using the terms ‘diabetes’, ‘cardiac’ and ‘fetus’. All studies comparing fetal cardiac function in diabetic pregnancies with normal pregnancies were selected. Articles were reviewed for diabetes type, therapy, glycemic control and ultrasound technique. Ultrasound data describing hypertrophy, diastolic, systolic or overall function were extracted. Results: 28 articles were included, representing data of 1487 patients and 1782 controls. Of the cases 808 had gestational diabetes (GDM), 602 had pre-existing diabetes (PDM) and 77 unknown. Ultrasound examination was performed in the 1st, 2nd or 3rd trimester in 3, 16 and 23 studies respectively. Cardiac hypertrophy, reported in 15 studies (n=919), was significantly more prevalent in diabetic pregnancy in 13/15 (87%) studies. Diastolic and systolic function was measured in 19 (n=1138) and 18 studies (n=1114) respectively. Diastolic function was significantly impaired in 14/19 (74%). Systolic function was decreased in 6/18 (33%) studies. 10 studies (n=441) measured overall function of which 8 (80%) found significant impairment. Forest plots of most used parameters – interventricular septum (IVS, n=648) and myocardial performance index (MPI, n=590) – showed significant increased IVS and MPI in diabetic group, with a mean difference of 0.73 [0.66, 0.79] (p<0.01) and 0.04 [0.04, 0.05] (p<0.01) respectively. Conclusions: Our review shows that diabetes in pregnancy can cause fetal cardiac hypertrophy and impaired cardiac function. This effect is more pronounced in PDM, but GDM also causes significant functional impairment. The effect is most visible in the 3rd trimester. Future research should focus on clinical importance of these findings for neonatal outcome. P19.03 Evaluation of emerging modalities for fetal global cardiac function assessment in normal pregnancies H. Avnet 1,2 , V. Leung 1 , J. Alphonse 1 , G. Beirne 1 , A.W. Welsh 1,2 1 School of Women’s and Children’s Health, University of New South Wales, Sydney, NSW, Australia; 2 Maternal-Fetal Medicine, Royal Hospital for Women, Sydney, NSW, Australia Objectives: Current fetal cardiac function ultrasound techniques have limited validation. Pulse-wave Doppler Myocardial Perfor- mance Index (PW-MPI), Tissue Doppler Imaging (TDI-MPI) and Tricuspid/Mitral Annular Plane Systolic Excursion (TAPSE/MAPSE) using spatiotemporal image correlation (STIC) M-mode are emerg- ing modalities, but are limited by small sample size and inconsistent results. This study aims to compare the clinical applicability of these three techniques to determine their future utility. Methods: A multi-modality, cross-sectional, prospective study. We recruited 79 uncomplicated singleton pregnancies at  The Authors 2017 214  Ultrasound in Obstetrics & Gynecology 2017; 50 (Suppl. 1): 154–256.