Volume 1 Number 2 p-ISSN: 2549-1849 | e-ISSN: 2549-3434 26 Received: 17 November 2017; Reviewed: 8 March 2018; Approved: 8 March 2018 http://jurnal.unimed.ac.id/2012/index.php/jcrs/article/view/9331 THE DIVERSITY Of FRUIT FLIES IN RED GUAVA CROPS AND PARASITOID PARASITIZATION LEVEL IN DISTRICT DELI SERDANG Putri Mustika Sari 1* , Darma Bakti 2 , Maryani Cyccu Tobing 2 1 Post Graduate of Agrothechnology, Faculty of Agriculture, University of Sumatera Utara, Medan, Indonesia. 2 Agrothechnology Department, Faculty of Agriculture, University of Sumatera Utara, Medan, Indonesia. *Corresponding Author: h0lm3s44@ymail.com Abstract Morphological and Molecular of Identification Bactrocera sp. (Diptera: Tephritidae) in 5 Location of Red Guava Crops District Deli Serdang. Fruit flies are a concern in the world because they are an important pest in fruits. Seven species of fruit flies (Bactrocera carambolae, Bactrocera papayae, Bactrocera curcubitae, Bactrocera caudata, Bactrocera albistrigata, Bactrocera tau, Bactrocera umbrosa) have been identified from the morphological identification of fruit flies in LIPI. The fruit fly is obtained from trapping with methyl eugenol, cocoa waste processing, and a mixture of both. Found 2 species of parasitoid (Psytallia sp. near Walker; Psytallia sp. near Walkeri) from the rearing of fruit that was attacked at five locations of red guava plantation district Deli Serdang. Keywords : Bactrocera spp., morphology, atractant, parasitoid 1. INTRODUCTION Fruit flies (Bactrocera sp.) are of concern in the world because they are an important pest in fruit, in Indonesia have experienced problems in fruits commodities (Suputa et al., 2007). Fruit farming can not be separated from pests, which can decrease production and become limiting factor of inter-country trade (Kardinan et al., 2009). Red guava productivity in District Deli Serdang is reported to have decreased since 2010. Red Guava production of 35.261 dropped to 12.661 tons in 2014 (BPS, 2016). Reduced productivity of red guava one of them can be caused by pest attacks that cause damage to the fruit, and decrease the quality and quantity of the harvest (Amin, 2015). That Bactrocera cucurbitae and B. papayae only attack papaya fruit only, while fruit flies that attack more than one fruit is B. carambolae attack mango fruit, starfruit, guava, guava water. B. albistrigata attack mango, guava, guava (Rahardjo et al., 2009). Various efforts to control fruit flies have been done among others by wrapping, sterile male insect technique, pesticide spraying, sanitation, and biological control (Dhillon et al ., 2005). One of the biological control that is by utilizing the role of parasitoid comes from family branconidae (Hymenoptera), namely Fopius sp. and Biosteres sp. which can suppress the population of fruit flies in the field (Siwi et al., 2006). Another fruit fly control that is effective and environmentally friendly is the use of trap atraktan (Kardinan et al., 2009). This study aims to identify the species that attack the red guava crops and parasitoid parasitation level in the district Deli Serdang 2. MATERIAL AND METHOD 2.1. Collection of Fruit Flies Collection of fruit fly samples using modified Steiner traps and attracted attractants. Traps are made using yellow painted plastic containers, and inside are placed cotton for place of attractant. Administering of attractant to cotton with syringes in accordance with each treatment and repeating each sampling. Trap mounting is done at 07.00 WIB. Trap is fitted with zig zag diagonal slice method which is randomly determining where fruit fly trap in red guava crops in each sampling location, where the distance between traps ± 20 m adjusted with different spacing in each location. The number of traps to be installed in each sample location is 9 pieces and the total trap throughout the sample location is 45 traps. The trap is suspended on a shaded tree (canopy) at an altitude of ± 1 - 1.5 m above ground level. The collection of fruit flies was done as much as 8x with weekly intervals. Trapped fruit flies were collected, inserted in a small bottle containing 70% alcohol, and given sample number, treatment, location, date of taking. Then samples were taken to the LIPI laboratory to be identified. 2.2. Intake of Infected Fruits Five fruits were attacked by purposive random sampling 4x with 2 weeks interval at each sample location. The collected fruit was inserted in plastic bag, labeled location, date of taking and taken to laboratory. The infected fruit was put into plastic container with sand media and covered with gauze. The fruit is observed after 7 days (6-10 day cycle of larvae), to get fruit fly pupa then sand diayaksetiapiapiap once for 14 days (pupa cycle 8-12 days). The