Agriprima, Journal of Applied Agricultural Sciences Maret, 2019 Online version : https://agriprima.polije.ac.id Vol. 3, No. 1, Hal. 54-61 P-ISSN : 2549-2934 | E-ISSN : 2549-2942 DOI: 10.25047/agriprima.v3i1.100 Publisher : Politeknik Negeri Jember 54 Uji Tujuh Genotipe Kedelai Generasi F7 Terhadap Ketahanan Serangan Karat Daun (Phakopsora pachyrhizi) Dengan Metode IWGSR Author(s): Rona Utama* (1) ; Nurul Sjamsijah (1) (1) Jurusan Produksi Pertanian, Politeknik Negeri Jember * Corresponding author: ronnarodly@gmail.com ABSTRAK Serangan karat daun yang disebabkan jamur Phakopsora pachyrhizi merupakan salah satu faktor yang menyebabkan penurunan produksi kedelai hingga 30 – 60%. Permasalahan ini menuntut pemuliaan untuk merakit varietas unggul baru yang tahan terhadap serangan karat daun. Penelitian ini dilakukan di lahan riset Politeknik Negeri Jember dari bulan Juni – Agustus 2017 menggunakan rancangan acak kelompok non faktorial dengan 3 kali ulangan menggunakan 14 genotipe yang terseleksi dari generasi F2 sampai F6. Keempatbelas genotipe tersebut yaitu 7 genotipe hasil persilangan; P3P2, P2P3, RD, P2D, P3R, P2R, P3D, 4 genotipe tetua; Dering, Rajabasa, Polije-2 dan Polije-3, dan 3 varietas pembanding yaitu Wilis, Malabar dan Ringgit. Parameter yang diuji nilai IWGSR (Internasional Working Group Of Soybean Rust), dan 3 komponen hasil (umur panen, berat 100 biji dan hasil pertanaman). Hasil test menunjukan genotipe P3P2, P2P3, P2R, P3R, P2D, RD, dan P3D memiliki kekebalan serangan karat daun dengan notasi R (Resistance), genotipe P2P3 memiliki umur panen yang genjah yaitu 68,53 HST, dan berat 100 biji pada genotipe RD memiliki hasil yang paling besar yaitu (14,542 g) dan genotipe P3D memiliki hasil (21,24 g) biji pertanaman. Kata Kunci: Genotipe unggul; Kedelai; Metode IWGSR; Karat Daun; Keywords: Superior Genotype; IWGSR Method; Soybean; Soybean Rust; ABSTRACT Factor that cause the decreasing of soybean production is disease attack that is caused by Phakopsora pachyrhizi which can reduce production by 30% - 60%. This conditions require plant breeders to assemble new improved varieties that are resistant to leaf rust attack. This research was conducted at research field State Polytechnic of Jember from June to August 2017 with the non factorial Randomized Complete Block Design (RCBD) in three replications. The research used 14 selected soybean genotypes from F2 to F6 generation. Seven genotypes were obtained from the crossing namely: P3P2, P2P3, P2D, RD, P3R , P2R, and P3D. Four genotypes namely: Dering, Rajabasa, Polije-2 and Polije-3 are the parental genotypes, and three genotypes used as the comparative varieties, are Wilis, Malabar and Ringgit. The International Working Group of Soybean Rust (IWGSR) method proposed parameters to observe including 3 components of yield (harvest age, weight of 100 seeds and crop yield). The results showed that the genotype of P3P2, P2P3, P2D, RD, P2R, P3R and P3D had resistance to leaf rust attack with R (Resistance) notation, P2P3 genotype had a shorter harvest age of 68,53 DAP, the weight of 100 seeds in RD genotype was 14,542 g, and the highest yield was shown by P3D genotype with 21,24 g seed per plant.