Veterinary Clinical Pathology, Vol.15, No. 57, Spring 2021 “Research article” DOI: 10.30495/JVCP.2020.670353.1165 Effect of Beta asarone on concentration of TNF-α in a rat model of Alzheimer’s disease Saki, G. 1,2 , Eidi, A. 3* , Mortazavi, P. 4 , Vahdati, A. 5,6 , Panahi, N. 7 1- PhD. Graduate, Department of Biology, Faculty of Basic Science, Fars Science and Research Branch, Islamic Azad University, Fars, Iran. 2- PhD. Graduate, Department of Biology, Faculty of Science, Agriculture and New Technologies, Shiraz Branch, Islamic Azad University, Shiraz, Iran. 3- Professor, Department of Biology, Faculty of Basic Science, Science and Research Branch, Islamic Azad University, Tehran, Iran. 4- Associate Professor, Department of Pathology, Faculty of Specialized Veterinary Sciences, Science and Research Branch, Islamic Azad University, Tehran, Iran. 5- Professor, Department of Biology, Faculty of Basic Science, Fars Science and Research Branch, Islamic Azad University, Fars, Iran. 6- Professor, Department of Biology, Faculty of Science, Agriculture and New Technologies, Shiraz Branch, Islamic Azad University, Shiraz, Iran. 7- Assistant Professor, Department of Basic Sciences, Faculty of Specialized Veterinary Sciences, Science and Research Branch, Islamic Azad University, Tehran, Iran. * Corresponding author’s email: eidi@srbiau.ac.ir (Received: 2020/5/7 Accepted: 2020/10/22) Abstract Beta asarone which is the major component of Acorus tatarinowii Schott, can pass through the blood-brain-barrier and affect the central nervous system. In the present study, effect of beta asarone on TNF-α level was investigated in β-amyloid-induced alzheimeric male rats. The adult male rats were randomly divided into 9 groups of 6: normal control, sham-operated control, β- asarone (12.5, 25 and 50 mg/kg PO, daily for 50 days), alzheimeric control (bilateral intrahippocampal injection of 4 μl of β-amyloid 1-42) and alzheimeric β-asarone receiving (12.5, 25 and 50 mg/kg PO β-asarone daily for 30 days following β-amyloid injection and subsequent doses of beta asarone for 3 weeks). The rats were sacrificed at the end of the experiment and the TNF-α level was measured in brain homogenate. Our results showed that administration of β-asarone (25 and 50 mg/kg) significantly decreased the TNF-α level (p<0.001) in alzheimeric rats. Thus, these results indicate that β-asarone is effective in providing protection against inflammation induced by β-amyloid. Conflict of interest: None declared. Keywords: Alzheimer, β-amyloid, β-asarone, Rat, Tumor Necrosis Factor-alpha.