www.afm-journal.de © 2021 Wiley-VCH GmbH 2108650 (1 of 21) REVIEW Engineered Nanoenzymes with Multifunctional Properties for Next-Generation Biological and Environmental Applications Puranjan Mishra, Junsang Lee, Deepak Kumar, Ricardo O. Lauro, Nazua Costa, Deepak Pathania, Smita Kumar, Jinwoo Lee,* and Lakhveer Singh* As a powerful tool, nanoenzyme electrocatalyst broadens the ways to explore bioinspired solutions to the world’s energy and environmental concerns. Eforts of fashioning novel nanoenzymes for efective electrode functionalization is generating innovative viable catalysts with high catalytic activity, low cost, high stability and versatility, and ease of production. High chemo-selectivity and broad functional group tolerance of nanoenzyme with an intrinsic enzyme like activity make them an excellent environmental tool. The catalytic activities and kinetics of nanoenzymes that beneft the development of nanoenzyme-based energy and environmental technologies by efectual electrode functionalization are discussed in this article. Further, a deep-insight on recent developments in the state-of-art of nanoenzymes either in terms of electrocatalytic redox reac- tions (viz. oxygen evolution reaction, oxygen reduction reaction, nitrogen reduc- tion reaction and hydrogen evolution reaction) or environmental remediation / treatment of wastewater/or monitoring of a variety of pollutants. The complex interdependence of the physicochemical properties and catalytic characteristics of nanoenzymes are discussed along with the exciting opportunities presented by nanomaterial-based core structures adorned with nanoparticle active-sites shell for enhanced catalytic processes. Thus, such modular architecture with multi-enzymatic potential introduces an immense scope of making its eco- nomical scale-up for multielectron-fuel or product recovery and multi-pollutant or pesticide remediation as reality. DOI: 10.1002/adfm.202108650 P. Mishra Institute of Bioresource and Agriculture Hong Kong Baptist University Kowloon Tong 852, Hong Kong J. Lee, J. Lee Department of Chemical and Biomolecular Engineering Korea Advanced Institute of Science and Technology (KAIST) 291 Daehak-Ro, Yuseong-Gu, Daejeon 34141, Republic of Korea E-mail: jwlee1@kaist.ac.kr D. Kumar Department of Chemical Engineering SUNY College of Environmental Science and Forestry Syracuse, NY 13210, USA R. O. Lauro, N. Costa Instituto de Tecnologia Química e Biológica António Xavier (ITQB-NOVA) Universidade Nova de Lisboa Oeiras 2780-157, Portugal D. Pathania Department of Environmental Science Central University of Jammu Bagla (Rahya-Suchani), Samba, Jammu & Kashmir 181143, India D. Pathania Department of Chemistry Sardar Vallabhbhai Patel Cluster University Mandi, Himachal Pradesh 175001, India S. Kumar Department of Environmental Sciences J. C. Bose University of Science & Technology YMCA, Sector-6, Mathura Road, Faridabad, Haryana 121006, India L. Singh Department of Environmental Science SRM University-AP Amaravati, Andhra Pradesh 522502, India E-mail: lakhveer.s@srmap.edu.in 1. Introduction Natural enzymes exhibit multi-dynamic catalytic activities which lead to improved cascade reactions in various biological systems. It owns efcient mass transfer activities which reduced the intermediate decomposition due to its local produc- tion at higher concentrations. [1] However, strict physiological conditions, selective stability, poor reusability for performing any particular catalytic reactions are the major intrinsic fragility of these naturally occurring enzymes. [2] In this pursuit, the expansions of nanotechnological advance- ment have led to the development of nanoscale materials that exactly mimic the “natural-enzymes” as well as exhibit sim- ilar intrinsic properties, popularly known as “nanoenzyme” or “nanoenzyme”. [3] As nanoenzymes, these nanomaterials pos- sess the ability to imitate various catalytic properties, which gradually sparked the researchers for nano-formulations and the discovery of a wide range of biomimetic compounds. Research about nanoenzyme The ORCID identifcation number(s) for the author(s) of this article can be found under https://doi.org/10.1002/adfm.202108650. Adv. Funct. Mater. 2021, 2108650