Research Article Manuka Honey Exerts Antioxidant and Anti-Inflammatory Activities That Promote Healing of Acetic Acid-Induced Gastric Ulcer in Rats Saad B. Almasaudi, 1 Aymn T. Abbas, 2,3 Rashad R. Al-Hindi, 1 Nagla A. El-Shitany, 4,5 Umama A. Abdel-dayem, 6 Soad S. Ali, 7 Rasha M. Saleh, 8 Soad K. Al Jaouni, 9 Mohammad Amjad Kamal, 10,11 and Steve M. Harakeh 12 1 Biology Department, Faculty of Science, King Abdulaziz University, Jeddah 21589, Saudi Arabia 2 Special Infectious Agents Unit, King Fahd Medical Research Center, King Abdulaziz University, Jeddah 21589, Saudi Arabia 3 Biotechnology Research Laboratories, Gastroenterology Surgery Center, Mansoura University, Mansoura 35516, Egypt 4 Department of Pharmacology and Toxicology, Faculty of Pharmacy, King Abdulaziz University, Jeddah 21589, Saudi Arabia 5 Department of Pharmacology and Toxicology, Faculty of Pharmacy, Tanta University, Tanta 31111, Egypt 6 Animal Facility Unit, King Fahd Medical Research Center, King Abdulaziz University, Jeddah 21589, Saudi Arabia 7 Anatomy Department (Cytology and Histology), Faculty of Medicine, King Abdulaziz University, Jeddah 21589, Saudi Arabia 8 Department of Animal Physiology, Faculty of Veterinary Medicine, Mansoura University, Mansoura 35516, Egypt 9 Department of Hematology and Yousef Abdulatif Jameel Chair of Prophetic Medicine Application, Faculty of Medicine, King Abdulaziz University, Jeddah 21589, Saudi Arabia 10 King Fahd Medical Research Center, King Abdulaziz University, Jeddah 21589, Saudi Arabia 11 Yousef Abdulatif Jameel Chair of Prophetic Medicine Application, Faculty of Medicine, King Abdulaziz University, Jeddah 21589, Saudi Arabia 12 Special Infectious Agents Unit, King Fahd Medical Research Center, Yousef Abdullatif Jameel Chair of Prophetic Medicine Application, King Abdulaziz University, Jeddah 21589, Saudi Arabia Correspondence should be addressed to Aymn T. Abbas; aymntalat2001@yahoo.co.uk Received 4 July 2016; Revised 30 October 2016; Accepted 3 November 2016; Published 19 January 2017 Academic Editor: Jos´ e Maur´ ıcio Sforcin Copyright © 2017 Saad B. Almasaudi et al. Tis is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Gastric ulcers are a major problem worldwide with no efective treatment. Te objective of this study was to evaluate the use of manuka honey in the treatment of acetic acid-induced chronic gastric ulcers in rats. Diferent groups of rats were treated with three diferent concentrations of honey. Stomachs were checked macroscopically for ulcerative lesions in the glandular mucosa and microscopically for histopathological alterations. Treatment with manuka honey signifcantly reduced the ulcer index and maintained the glycoprotein content. It also reduced the mucosal myeloperoxidase activity, lipid peroxidation (MDA), and the infammatory cytokines (TNF-, IL-1, and IL-6) as compared to untreated control group. In addition, honey-treated groups showed signifcant increase in enzymatic (GPx and SOD) and nonenzymatic (GSH) antioxidants besides levels of the anti- infammatory cytokine IL-10. Flow cytometry studies showed that treatment of animals with manuka honey has normalized cell cycle distribution and signifcantly lowered apoptosis in gastric mucosa. In conclusion, the results indicated that manuka honey is efective in the treatment of chronic ulcer and preservation of mucosal glycoproteins. Its efects are due to its antioxidant and anti- infammatory properties that resulted in a signifcant reduction of the gastric mucosal MDA, TNF-, IL-1, and IL-6 and caused an elevation in IL-10 levels. Hindawi Evidence-Based Complementary and Alternative Medicine Volume 2017, Article ID 5413917, 12 pages https://doi.org/10.1155/2017/5413917