Siver Nanoparticles Toxicity in Brine Shrimp and its Histopathological Analysis Supicha Kachenton * , Varanut Whangpurikul † , Niwat Kangwanrangsan * , Tawewan Tansatit ‡ and Wannee Jiraungkoorskul * ,§ * Department of Pathobiology, Faculty of Science Mahidol University, Bangkok 10400, Thailand † Mahidol University International College, Mahidol University Nakhon Pathom 73170, Thailand ‡ Department of Preclinical and Applied Animal Science Faculty of Veterinary Science, Mahidol University Nakhon Pathom 73170, Thailand § wannee.jir@mahidol.ac.th Received 29 December 2016 Accepted 26 September 2017 Published 29 January 2018 Silver nanoparticles (Ag NPs) are interesting nanotechnology materials with borderless applications in medical science, environmental science, material science and are also used in various kinds of industrial household products. Increasing usage of Ag NPs every year leads to increased risk of nanomaterial contamination in the environment, especially natural water sources with harmful e®ects in aquatic animals, and to ecosystem disruption. In the present study, Ag NPs toxicity was determined using brine shrimp Artemia salina as a model. A total of 10 adult brine shrimps were incubated in solutions containing various Ag NPs concentrations for 24 h at room temperature. Percentages of brine shrimp dead were recorded and calculated to determine the lethal concentration. We found the LC 50 of Ag NPs is 3521.13 mg/L. Histopa- thology study was done using survival brine shrimps after 24 h of incubation with 25% LC 50 Ag NPs concentration. Tissue processing and H&E staining to remark pathological areas with emphasis on intestinal tract, were also done. Ag NPs obstruction was found in intestinal lumen. The intestinal epithelial cells showed hyperplasia and blebbing, increased mucous thickening, detachment from basement muscle lining, and necrosis area. These ¯ndings indicated the e®ect of Ag NPs and their negative impact on aquatic animals which might lead to further disruption of ecosystem. Keywords : Artemia salina; histopathology; median lethal concentration; silver nanoparticles; toxicology. 1. Introduction Nanoparticles originate from nanotechnology, and have become increasingly interesting materials due to their wide applications in various ¯elds in- cluding agriculture, 1 biology, 2 biomedicine, 3,4 food science, 5 engineering 6 and physics. 7 By de¯nition, International Journal of Nanoscience Vol. 17, No. 6 (2018) 1850007 (5 pages) # . c World Scienti¯c Publishing Company DOI: 10.1142/S0219581X18500072 1850007-1 Int. J. Nanosci. 2018.17. Downloaded from www.worldscientific.com by 181.215.126.13 on 08/08/19. Re-use and distribution is strictly not permitted, except for Open Access articles.