Agricultural and Forest Meteorology 111 (2002) 171–186 Automated closed-system canopy-chamber for continuous field-crop monitoring of CO 2 and H 2 O fluxes P. Steduto a,* , Ö. Çetinkökü a , R. Albrizio a , R. Kanber b a CIHEAM-IAMB, via Ceglie 9, 70010 Valenzano (BA), Italy b Çukurova University, Adana, Turkey Received 30 July 2001; received in revised form 12 March 2002; accepted 22 March 2002 Abstract An automated transient-state closed-system canopy-chamber for gas-exchange determinations of field-crops was developed to allow unattended day and night, high frequency CO 2 and H 2 O exchange measurements, with short measurement time (15 s) and high scanning rate (0.5 s). Environment tests on the chamber performance indicated that: atmospheric pressure variation during operation was 10–20 Pa and limited to a few seconds; leaks introduced maximum errors of 1% in the flux calculation; turbulence effect on CO 2 and H 2 O fluxes was not detectable; during the measurement period, temperature build-up inside the chamber was typically within 1–2 C range; photosynthetic photon flux density (R p ) attenuation by the chamber as a whole was in the 15–20% range; whereas, net radiation (R n ) inside the chamber was always higher than outside by 10–20%. The flux determinations of the automated canopy-chamber were compared with other methods. Daily evapotranspiration (E) of artichoke obtained by canopy-chamber was at the most 4.2% lower than E obtained by high-precision weighing lysimeter. Diurnal E and carbon exchange rates (A) of sugarbeet and marjoram crops obtained by canopy-chamber closely followed the pattern of those obtained by the Bowen-ratio/energy-balance (BREB) method, with maximum daily underestimations of 6–8 and 5–6% for E and A, respectively. No differences in flux calculations came out when the rate of change in CO 2 and H 2 O concentrations was derived either by linear or quadratic regressions (QR). The results of all tests and comparisons showed that the automated chamber presented is a valuable and accurate tool for monitoring day and night CO 2 and H 2 O fluxes. © 2002 Elsevier Science B.V. All rights reserved. Keywords: Canopy-chamber; Gas-exchange; Evapotranspiration; Carbon exchange rate 1. Introduction Accurate determinations of CO 2 and H 2 O fluxes of field-crops are needed for various eco-physiological investigations. Photosynthesis, dark respiration and evapotranspiration responses of crops under differ- ent experimental conditions are, indeed, crucial for the understanding of growth and productivity and Corresponding author. Tel.: +39-080-4606224; fax: +39-080-4606206. E-mail address: steduto@iamb.it (P. Steduto). supply valuable information for crop modeling as well. Several methods can be used for concurrent mea- surements of water vapor and CO 2 fluxes, each with their advantages and limitations. Micrometeorological methods (Baldocchi et al., 1988) have the advantages of not disturbing the environment around the plant canopy, although their large spatial-scale requirement is not suited for typical agronomic plot experiments (Sharma, 1984). Canopy-chamber methods remain the sole approach for plot-sized experimental agriculture. Two major 0168-1923/02/$ – see front matter © 2002 Elsevier Science B.V. All rights reserved. PII:S0168-1923(02)00023-0