Int.J.Curr.Microbiol.App.Sci (2017) 6(10): 780-789 780 Original Research Article https://doi.org/10.20546/ijcmas.2017.610.094 Evaluation of Anthelmintic Efficacy of Nicotiana tabacum against Gastrointestinal Nematodes of Goats Sushmita Sastya, Rajeev Ranajan Kumar* and Stuti Vatsya College of Veterinary and Animal Sciences, G.B. Pant University of Agriculture and Technology, Pantnagar-263145, Uttarakhand, India *Corresponding author ABSTRACT Introduction Sheep and goats serve as major source of income for poor and landless farmers of Indian subcontinent, but the production performance of these animals is drastically reduced due to parasitic infections. Sheep and goats are highly susceptible to various helminth infections specially goats due to its unique grazing behaviour. Among nematodes, Haemonchus contortus is the most pathogenic gastrointestinal (GI) nematode distributed in different agro-climatic regions throughout the year. It causes heavy economic losses in terms of retarded growth, weight loss, lowered productivity and even mortality (Tyasi and Tyasi, 2015). Several methods have been employed for controlling the International Journal of Current Microbiology and Applied Sciences ISSN: 2319-7706 Volume 6 Number 10 (2017) pp. 780-789 Journal homepage: http://www.ijcmas.com The present study was planned to evaluate the anthelmintic efficacy of crude powder (CP), crude aqueous (CAE) and crude methanolic (CME) extracts of Nicotiana tabacum leaves against common gastrointestinal nematodes of goats using three in-vitro tests viz. adult motility test (AMT), egg hatch assay (EHA) and larval paralysis test (LPT) and one in- vivo test using faecal egg count reduction test (FECRT). AMT was conducted against freshly collected adult Haemonchus contortus. However, EHA and LPT tests were conducted against eggs and larvae of gastrointestinal nematodes, respectively of goats or AMT at different concentrations viz.0.156%, 0.312%, 0.625%, 1.25%, 2.5%, 5% and 10%. In-vivo trial was also conducted in goats naturally infected with mixed infection of GI nematodes using FECRT. In AMT, an average highest corrected motility of 100% was recorded from 0.625–10% concentration followed by 66.66% at 0.156 and 0.312% concentrations. Overall CP and CAE showed maximum (100%) adulticidal activity against H. contortus and minimum (71.43%) with CME. In EHA, CME of N. tabacum showed better ED 50 =0.522 and ED 99 = 21.32mg/ml in comparison to CAE (ED 50 =0.40 and ED 99 = 36.22mg/ml). The highest (100%) inhibition of egg hatching was observed with CAE @ 50 and 100mg/ml and CME @ 25, 50 and 100 mg/ml concentration. In case of LPT, crude methanolic extract has better (ED 50 = 0.69 and ED 99 = 9.58 mg/ml) values than crude aqueous extract (ED 50 = 0.759 and ED 99 = 14.9349 mg/ml). The highest (100%) paralysis of third stage larvae was observed with CAE @ 25, 50 and 100mg/ml and CME @12.5, 25, 50 and 100mg/ml concentration. In in-vivo trial, the highest (100%) reduction in faecal egg count was recorded against CAE @ both 100 and 200mg/kg b. wt. 14 days post-treatment. However, CME and CP both showed maximum effect of 33.33% and 15.79%, respectively @200mg/kg b. wt. on 14 days post-treatment. Keywords Nicotiana tabacum, Anthelmintic activity, GI nematodosis, Goats. Accepted: 07 September 2017 Available Online: 10 October 2017 Article Info