Volume 3 Number 2 p-ISSN: 2549-1849 | e-ISSN: 2549-3434 http://jurnal.unimed.ac.id/2012/index.php/jcrs/article/view/12153 Received: 10 July 2019; Accepted to be Published: 17 December 2019 89 COMBINED EFFECT OF STREPTOMYCIN AND SWEET ORANGE ESSENTIAL OIL TO MEMBRANE AND ACROSOME INTEGRITY BOER GOAT FROZEN SEMEN Sukma Aditya Sitepu 1 *, Julia Marisa 2 1 Department of Animal Husbandry, Faculty of Sains and Technology, Pembangunan Panca Budi University, Medan, Indonesia 2 Department of Agroecotechnology, Faculty of Sains and Technology, Pembangunan Panca Budi University, Medan, Indonesia *Corresponding author: sukmaaditya@dosen.pancabudi.ac.id Abstract One of factors that cause a bad quality of Boer Goat frozen semen is the growth of bacterial. This can be overcome by adding antibiotics such as streptomycin. To further suppress the growth of bacteria can be added other ingredients that contain antibacterials such as sweet orange essential oil. The purpose of this research is to know the percentage value of Membrane Integrity and Acrosome Integrity on Boer Goat frozen semen with addition sweet orange essential oil and streptomycin. The method used was experimental using Completely Randomized Design with 5 treatments and 5 replications. The treatment in this research is addition 0%, 0,25%; 0.5%; 0.75% and 1% sweet orange essential oil on tris yolk and streptomycin extender. The results showed the best treatment addition combination streptomycin and sweet orange essential oil to percentage Membrane Integrity and Acrosome Integrity is increase 1% sweet orange essential oil. Keywords: Boer Goat, essential oil, frozen semen, streptomycin, sweet orange. 1. INTRODUCTION Goat meat demand is high enough to meet the domestic needs and for export. Therefore it is necessary to consider how to improve the population and genetic quality of local goats. Local goats have low body weight so that the meat produced is also small so it is not enough to meet domestic needs and does not meet export requirements abroad. The weight of local goat bodies should be increased so that domestic meat needs can be met and can be exported. One way to improve the local goat's body weight is to do a cross breeding using superior goat, as Boer Goat. The Boer Goat is one of the superior goats that can be used to improve the genetic quality of local goats. Boer Goats have several advantages of body weight and high litter size. Local goat Indonesia although it has a low body weight but has the advantage as able to adapt to the extreme environment so that the results of the cross is expected to produce good quality goats. The obstacle is very difficult to get a good quality Boer Goat. In addition, maintenance and feed costs to be provided are also very expensive. Artificial Insemination is the best solution for being able to cross a local goat with a Boer Goat. However, many farmers have not understood about Artificial Insemination in goats (Marisa and Sitepu, 2018). By doing Artificial Insemination breeders do not need to maintain a Boer Goat that is expensive. One Boer Goat can produce sperm that is used for many local doe goat in a one ejaculation. Another advantage of Artificial Insemination is to minimize the danger of disease transmission and good semen quality because it comes from a superior goat. Goat farm in Indonesia spread to many areas difficult to reach because its distance from downtown and bad road conditions. Therefore, the use of Boer Goat Liquid semen in Artificial Insemination can not be done. Boer liquid cement is not durable, so it can not be used to carry out artificial Insemination in distant places. Liquid cement can be processed into frozen semen in order to be stored for a long time and can be sent to distant areas. Frozen semen that is stored and frozen in containers can still be used for decades. The success rate of Artificial Insemination in goats is influenced by the quality of frozen semen. One of the causes of the low quality of Boer Goat frozen semen is the development of bacteria.To inhibit bacterial growth in frozen semen can be done by adding antibiotics.The antibiotic that is often used in the manufacture of frozen semen is streptomycin. Provision of streptomycin in diluent semen has been done.But the results obtained are still not good so need to be done another way to suppress the growth of bacteria.The effort that can be done is to combine antibiotics in the diluent.Combination of antibiotics on cement diluents has been done by combining with several other antibiotics such