International Journal of Applied Chemistry. ISSN 0973-1792 Volume 13, Number 3 (2017) pp. 707-720 © Research India Publications http://www.ripublication.com Study Biodegradation of Aromatics Pyrene Using Bacterial Isolates from the Sea and micro symbionts Sponges Ismail Marzuki 1* , Hadija Enryani Ismail 2 , Nursiah La Nafie 3 and Seniwati Dali 4 1 Basic Laboratory of Chemical Engineering, Faculty of Engineering, University of Fajar, Indonesia 2,3 Laboratory of Analytical Chemistry, Department of Chemistry, Faculty of Mathematics and Natural Sciences, University of Hasanuddin, Indonesia 4 Biochemistry Laboratory, Department of Chemistry, Faculty of Mathematics and Natural Sciences, University of Hasanuddin, Indonesia. Correspondint author’s email: ismailmz@unifa.ac.id; cc ismailmz3773@gmail.com Abstract Investigation of PAHs reshuffle by microorganisms has been done, but using sponge symbionts for the purpose of reduction of PAHs toxicity into simple organic compounds is still small. Microorganisms and sponges are known as complex animals that have various functions including the ability to produce bioactive compounds that can interact with hydrocarbon compounds as carbon sources. The purpose of this experiment was the use of bacteria isolated from seawater and sponges to reduce pyrene by biodegradation method by interacting between bacteria Alcaligenes faecalis strain Cu4-1 (AF), Bacillus subtilis strain BAB-1684 (BS) and Bacillus Cereus strain MER-8 (BC) capable of producing enzymes to overhaul the pyrene structure acting as a substrate. Biodegradation was carried out for 25 days, every 5 days to check degradation media using spectrophotometer and GC-MS to analyze degradation parameters: (1) optical density, (2) component abundance, (3) concentration and degradation rate of pyrene, and (4) ) Degraded organic of components. The results of the analysis of each degradation parameter were concluded: isolates, AF, BS and BC were able to degrade pyrene compounds, biodegradation rate: AF (97, 65%) ˃ BC (95, 27%) ˃ BS (93, 15%). Marine-filled bacteria (AF and BC) have a relatively