Korean J Clin Lab Sci. Vol. 50, No. 3, September 2018 275 www.kjcls.org ORIGINAL ARTICLE Korean J Clin Lab Sci. 2018;50(3):275-283 https://doi.org/10.15324/kjcls.2018.50.3.275 pISSN 1738-3544 eISSN 2288-1662 Comparative Proteome Analysis of Zerumbone-treated Helicobacter pylori Sa-Hyun Kim Department of Clinical Laboratory Science, Semyung University, Jecheon, Korea Zerumbone 처리에 따른 Helicobacter pylori의 단백질 비교분석 김사현 세명대학교 임상리학과 Helicobacter pylori is a causative organism of various gastrointestinal diseases, including chronic gastritis, gastric ulcer, or gastric adenocarcinoma. Pathogenic factors, such as cytotoxin- associated protein A (CagA) and vacuolating cytotoxic protein A (VacA), play a role. This study analyzed qualitatively and quantitatively the effects of zerumbone on the changes in the protein expression levels of various H. pylori proteins, including CagA and VacA. Approximately 200 significant proteins were screened for the H. pylori 60190 (VacA positive / CagA positive; Eastern type) strain, and proteomic analysis was performed on 13 protein molecules that were clinically significant. After two-dimensional electrophoresis (2-DE), ImageMaster TM 2-DE Platinum software was used for quantitative measurements of protein spots. Matrix-assisted laser desorption/ ionization-mass spectrometry (MALDI-TOF-MS) and liquid chromatography–mass spectrometry/ mass spectrometry (LC-MS/MS) were used for protein identification. After intensive analysis of the proteins that showed significant changes, a reverse transcription-polymerase chain reaction was performed as required to verify the results. In this study, the significance of zerumbone as a therapeutic agent for H. pylori infection was examined by screening a new pharmacological activity mechanism of zerumbone. Key words: AmiE, CagA, Helicobacter pylori, OorA, VacA Corresponding author: Sa-Hyun Kim Department of Clinical Laboratory Science, Semyung University, 65 Semyeong-ro, Jecheon 27136, Korea Tel: 82-43-649-1624 Fax: 82-50-4411-9604 E-mail: science4us@semyung.ac.kr This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. Copyright © 2018 The Korean Society for Clinical Laboratory Science. All rights reserved. Received: August 11, 2018 Revised 1 st : August 15, 2018 Revised 2 nd : August 20, 2018 Revised 3 rd : August 24, 2018 Revised 4 th : August 29, 2018 Accepted: August 30, 2018 서 론 현재까지 질환과 원성 인자 사이의 연관성을 규명하기 위 하여 다양한 Helicobacter pylori 임상균주를 대상으로 프로테 오믹스 분석 결과가 고되어 왔다. 무엇다도 Cytotoxin- associated protein A (CagA)와 Vacuolating cytotoxic protein A (VacA)가 위염 및 위암 과 관련이 큰 H. pylori의 가장 대표적인 원성 인자로 알려져있다[1]. 특히 CagA의 경 우, cag pathogenicity island (cag PAI) 내에 위치한 유전자의 열에 따라 H. pylori의 원성이 결정되는 것으로 판단되며, 위암 에 있어 가장 중요한 원성 결정인자이다[2-4]. Urease, flagella, adhesin 및 ferritin 또한 H. pylori의 원성 결정 인자로서 고되고 있다[5-7]. 현재 H. pylori의 원성 인 자 파악에 있어서 26695와 J99 균주의 경우에는 완전한 게놈 서