Complete Genome Sequence of Xanthomonas citri pv. anacardii Strain IBSBF2579 from Brazil Wilson José Silva Junior, a Antonio Roberto Gomes Farias, a Nelson Bernardi Lima, a Ana Maria Benko-Iseppon, b Flávia Aburjaile, b Valdir Queiroz Balbino, b Raul Maia Falcão, b Sérgio de Sa Leitão Paiva Júnior, b Lucas Christian Sousa-Paula, b Rosa Lima Ramos Mariano, a Elineide Barbosa Souza, c Marco Aurélio Siqueira Gama a a Department of Agronomy, Federal Rural University of Pernambuco, Recife, Brazil b Department of Genetics, Federal University of Pernambuco, Recife, Brazil c Department of Biology, Federal Rural University of Pernambuco, Recife, Brazil ABSTRACT The bacterium Xanthomonas citri pv. anacardii is the agent of angular leaf spot of the cashew tree (Anacardium occidentale L.). The complete genome se- quencing of the strain IBSBF2579 was done on an Illumina HiSeq 2500 platform. The de novo assembly of the X. citri pv. anacardii strain IBSBF2579 genome yielded 133 contigs, with a size of 5,329,247 bp and a G+C content of 64.03%. The prediction was performed by GeneMarkS and the automatic annotation by Rapid Annotations using Subsystems Technology (RAST), with 4,406 identified genes. T he cashew tree is native to Brazil (1) and grows in South America’s tropical regions, mainly in the northeast of the country (2). Cashew nut production may be impaired by the occurrence of diseases, such as standout angular leaf spot caused by Xanthomo- nas citri pv. anacardii and X. citri pv. mangiferaeindicae (3, 4). These bacteria show pathological differences in cashew and mango trees, being able to multiply and survive in different hosts in the Anacardiaceae family (5, 6). However, until now, only X. citri pv. mangiferaeindicae (LMG 941) has been sequenced (7). Here, we report the whole- genome sequence of the type strain of X. citri pv. anacardii (IBSBF2579 = ICMP4088), which is nonpigmented and was isolated from a cashew tree in Brazil. The genome sequencing was performed by using the Illumina HiSeq 2500 platform at the Functional Genomics Center of the University of São Paulo. The libraries were prepared with the Illumina Nextera XT DNA library prep kit v 4, and sequencing was performed on a HiSeq flow cell v4, with the HiSeq SBS kit v4 and paired reads of 100 bp (2). Initially, the quality reads were analyzed by FastQC (8), and read trimming to remove the adapters was performed by using FASTX-Toolkit (v. 0.0.13) (9). The genome de novo assembly was carried out using SPAdes (v. 1.10) (10), yielding 133 contigs of 500 bp (N 50 , 150,594 bp), with the largest contig being 344,686 bp, for a total assembly size of 5,329,247 bp with a G+C content of 64.03%. The annotation using GeneMarkS (11) predicted 4,406 genes by Rapid Annotations using Subsystems Technology (RAST) (12). All assembly statistics were generated with QUAST (v.4.6) (13). Accession number(s). This whole-genome shotgun project has been deposited at DDBJ/ENA/GenBank under the accession number PESI00000000. The version described in this paper is version PESI01000000. ACKNOWLEDGMENTS This study was supported by the Coordination for Improvement of Personnel with Higher Education (CAPES) Computational Biology Program and fellowships from the National Council for Scientific and Technological Development (CNPq) for the scholar- ships awarded to A.M.B.-I. (310871/2014-0), E.B.S. (305843-2016-8), and V.Q.B. (312002/2015-7). Received 17 December 2017 Accepted 19 December 2017 Published 1 February 2018 Citation Silva Junior WJ, Farias ARG, Lima NB, Benko-Iseppon AM, Aburjaile F, Balbino VQ, Falcão RM, Paiva Júnior SDSL, Sousa-Paula LC, Mariano RLR, Souza EB, Gama MAS. 2018. Complete genome sequence of Xanthomonas citri pv. anacardii strain IBSBF2579 from Brazil. Genome Announc 6:e01574-17. https://doi .org/10.1128/genomeA.01574-17. Copyright © 2018 Silva Junior et al. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license. Address correspondence to Marco Aurélio Siqueira Gama, mas.gama@yahoo.com.br. PROKARYOTES crossm Volume 6 Issue 5 e01574-17 genomea.asm.org 1