Korean J Hematol Vol. 42, No. 4, December, 2007 □ Original Article □ 미세 유체 분배 기법을 이용한 혈액형 검사 키트의 개발 1 강원대학교 의과대학 진단검사의학교실, 2 (주) 디지털바이오테크놀러지, 3 서울대학교 공과대학 기계항공공학부, 4 전기컴퓨터공학부, 5 고려대학교 의과대학 진단검사의학교실 서인범 1 ㆍ유숙원 1 ㆍ이용구 2,3 ㆍ허대성 2 ㆍ정찬일 2 ㆍ장준근 2,4 ㆍ임채승 5  Development of a New Blood Typing Kit Using the Microfluidics Separation Technique In Bum Suh, M.D. 1 , Sook Won Ryu, M.D. 1 , Yongku Lee 2,3 , Dae Sung Hur 2 , Chanil Chung, Ph.D. 2 , Jun Keun Chang, Ph.D. 2,4 and Chae Seung Lim, M.D. 5 1 Department of Laboratory Medicine, Kangwon National University College of Medicine, Chuncheon, 2 Digital Bio Technology Co, 3 Seoul National University School of Mechanical and Aerospace Engineering, 4 Seoul National University School of Electrical Engineering and Computer Science, 5 Department of Laboratory Medicine, Korea University College of Medicine, Seoul, Korea Background: Blood typing is an essential test for transfusion. Generally, blood typing is performed using a slide test, tube test or microcolumn agglutination test. The aims of this study were to develop a new blood typing kit using micromachining, microfluidics and microseparation methods, and to evaluate the clinical usefulness of the new blood typing kit. Methods: We designed and manufactured a blood typing microchip using polydimethylsiloxane (PDMS), which contained a microchannel (25∼200μm). The blood sample and antisera to be tested were dropped on the microwell for movement and mixing by capillary action. Once agglutination occurred, the micro- channel acts as a filter and the blood type was determined by observation by the naked eye. To evaluate the newtyping kit, we tested sensitivity using artificially diluted blood and compared the results of the new typing method with the slide and tube methods using 70 samples. Results: The new blood typing kit could differentiate a +4∼+2 agglutination reaction, but could not detect a +1 agglutination reaction as observed by the naked eye. Among 70 samples, the results of ABO and Rh typing by the new typing method (n=66, ≥+2 agglutination reaction by the column agglutination method) were in accord with the results of the tube and slide methods, but couldnot detect agglutination in all 4 clinical samples, below a +1 agglutination reaction. Conclusion: The new blood typing kit is inadequate for routine use in the clinical laboratory due to low sensitivity, but with further improvement, it can be used economically, conveniently and objectively for blood typing without any special equipment. Moreover, the microfludics and separation method may be broadly applicable in other tests using the hemagglutination method. (Korean J Hematol 2007;42: 392-396.)  Key Words: ABO, Rh, Blood typing, Microfluidics, Microseparation 392 ：2007 10 15 , ：2007 12 13 ：2007 12 17 ： , 516  452-020, Tel: 031-412-5300, Fax: 031-412-5314 E-mail: malarim@korea.ac.kr 2002 . Correspondence to：Chae Seung Lim, M.D. Department of Laboratory Medicine, Korea University College of Medicine 516, Gojan-dong, Danwon-gu, Ansan 452-020, Korea Tel: ＋82-31-412-5300, Fax: ＋82-31-412-5314 E-mail: malarim@korea.ac.kr