_____________________________________________________________________________________________________ *Corresponding author: E-mail: asashehabi2@gmail.com; Journal of Pharmaceutical Research International 23(6): 1-9, 2018; Article no.JPRI.44636 ISSN: 2456-9119 (Past name: British Journal of Pharmaceutical Research, Past ISSN: 2231-2919, NLM ID: 101631759) Detection Probiotic's DNA of Lactobacillus paracasei in Healthy Human Faeces Baraa M. Altaha 1 , Jamal Wadi 2 and Asem A. Shehabi 1* 1 Department of Pathology Microbiology, School of Medicine, The Jordan University, Amman, Jordan. 2 Division of Infectious Diseases, Department of Internal Medicine, School of Medicine, Jordan University Hospital, Amman, Jordan. Authors’ contributions This work was carried in collaboration between all authors who have designed the study. Author BMA has done all the laboratory tests, performed the statistical analysis and wrote the first draft of the manuscript. Author JW supervised the clinical part and author AAS supervised the laboratory tests. All authors read and approved the final study. Article Information DOI: 10.9734/JPRI/2018/44636 Editor(s): (1) Dr. Emmanuel Christy Jeyaseelan, Department of Botany, University of Jaffna, Sri Lanka. Reviewers: (1) R. Prabha, Dairy Science College, KVAFSU, India. (2) Maria De Lourdes Reyes Escogido, Universidad De Guanajuato, Mexico. (3) Olagoke, Olaniran Victor, Obafemi Awolowo University, Management Training Institute (ARMTI), Nigeria. Complete Peer review History: http://www.sciencedomain.org/review-history/26700 Received 22 July 2018 Accepted 10 October 2018 Published 19 October 2018 ABSTRACT Aims: To examine the DNA abundance of the probiotic bacteria (Lactobacillus paracasei) in the faeces of healthy adults after one month of its consumption and to determine the antimicrobial susceptibility profile of this bacteria. Study Design: Thirty apparently healthy adults were examined for the presence of probiotic bacteria DNA in their faecal samples over a period of one month after one week of probiotic consumption. Place and Duration: Department of Pathology and Microbiology, School of Medicine, The University of Jordan, Amman, Jordan, Between July 2017 and January 2018 Results: L. paracasei DNA detected in 90% of these adults within a week of probiotic consumption, whereas after stopping the probiotic consumption, L. paracasei DNA was detected only in 10% and 6% of the faecal samples after one and two weeks, respectively. Minimal side effects were recorded among these volunteers’ adults. L. paracasei was susceptible to ampicillin, chloramphenicol, clindamycin, erythromycin, imipenem and piperacillin–tazobactam, intermediate Original Research Article