DTD 5 Announcement of population data Allele frequencies for 27 Y-STR loci with U.S. Caucasian, African American, and Hispanic samples John M. Butler * , Amy E. Decker, Peter M. Vallone, Margaret C. Kline Biotechnology Division, National Institute of Standards and Technology, Gaithersburg, MD 20899-8311, USA Received 26 January 2005; received in revised form 22 February 2005; accepted 22 February 2005 Abstract A total of 263 U.S. Caucasians, 260 African Americans and 140 U.S. Hispanics or a subset of 31 Caucasians, 32 African Americans, and 32 Hispanics were typed for 27 Y-chromosome short tandem repeat (Y-STR) markers: DYS444, DYS446, DYS449, DYS463, DYS485, DYS490, DYS495, DYS504, DYS505, DYS508, DYS520, DYS522, DYS525, DYS532, DYS533, DYS534, DYS540, DYS556, DYS557, DYS570, DYS575, DYS576, DYS594, DYS632, DYS635, DYS641, and DYS643. Allele frequencies for each locus are reported along with nomenclature based on sequence analysis. # 2005 Elsevier Ireland Ltd. All rights reserved. Keywords: Short tandem repeat; DNA; Y-chromosome; Y-STR; Population data; DYS444; DYS446; DYS449; DYS463; DYS485; DYS490; DYS495; DYS504; DYS505; DYS508; DYS520; DYS522; DYS525; DYS532; DYS533; DYS534; DYS540; DYS556; DYS557; DYS570; DYS575; DYS576; DYS594; DYS632; DYS635; DYS641; DYS643; Y-GATA-C4; U.S. Caucasian; African American; U.S. Hispanic Population samples: Anonymous liquid blood samples with self-identified ethnicities were purchased from Inter- state Blood Bank, Inc. (Memphis, TN) and Millennium Biotech, Inc. (Ft. Lauderdale, FL). All samples were pre- viously examined with 15 autosomal short tandem repeats and the amelogenin sex-typing marker using the AmpFlSTR Identifiler kit (Applied Biosystems, Foster City, CA) to verify that each sample was unique [1]. N: Two hundred and sixty U.S. Caucasians, 260 African Americans, and 140 U.S. Hispanics were typed for 27 Y- chromosome short tandem repeat (Y-STR) markers. For six of the loci only a subset of each population group was examined that included 31 Caucasians, 32 African Amer- icans, and 32 Hispanics. DNA extraction: Blood samples were extracted using a modified salting out procedure [2] as described previously [1]. Quantification: Extracted DNA was quantified using UV spectrophotometry followed by a PicoGreen assay [3] to adjust concentrations to approximately 1 ng/mL (see [1]). Y-STR markers: Table 1 lists information on the 27 Y- STR markers examined as part of this study (see also http:// www.cstl.nist.gov/biotech/strbase/y_strs.htm). The PCR pri- mer sequences used in this study were those present in the Genome Database (GDB; http://www.gdb.org). In an attempt to generate smaller PCR products for some of the Y-STR loci, new primers were designed for DYS485 (129 bp), DYS490 (38 bp), and DYS495 (77 bp) with the size reduction relative to the GDB generated amplicons shown in parentheses. Fluorescent dyes 6-FAM, VIC, or NED were included on the 5 0 -end of the forward primers to create blue, green, or yellow-labeled PCR products. The reverse primers included an extra seven base tail (shown in lower case letters) to promote non-template addition. The DYS635 (Y-GATA-C4) data were obtained as part of an evaluation of a beta-test version of the Yfiler kit (Applied Biosystems). The exact chromosomal locations were ascer- tained using BLAT (http://genome.ucsc.edu/cgi-bin/hgBlat) www.elsevier.com/locate/forsciint Forensic Science International xxx (2005) xxx–xxx * Corresponding author. Tel.: +1 301 975 4049; fax: +1 301 975 8505. E-mail address: john.butler@nist.gov (J.M. Butler). 0379-0738/$ – see front matter # 2005 Elsevier Ireland Ltd. All rights reserved. doi:10.1016/j.forsciint.2005.02.011