Research Article
Effect of Bacillus cohnii on Some Physicomechanical and
Microstructural Properties of Ordinary Portland Cement
Ngari Reginah Wangui ,
1
Joseph Karanja Thiong’o,
1
and Jackson Muthengia Wachira
2
1
Department of Chemistry, Kenyatta University, Nairobi, Kenya
2
Department of Physical Sciences, University of Embu, Embu, Kenya
Correspondence should be addressed to Ngari Reginah Wangui; ngarireginah@gmail.com
Received 8 August 2020; Revised 7 September 2020; Accepted 14 September 2020; Published 27 September 2020
Academic Editor: Nenad Ignjatovi
Copyright©2020NgariReginahWanguietal.isisanopenaccessarticledistributedundertheCreativeCommonsAttribution
License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is
properly cited.
Cement-made materials face durability and sustainability challenges. is is majorly caused by the presence of cracks. Cracking
affects the mechanical strength of cement-based materials. Microbiologically induced calcite precipitation (MICP) has been found
to enhance compressive strength, thus enhancing on the mechanical and durability properties of these materials. is paper
presents the findings of a study conducted to investigate the effect of Bacilluscohnii on compressive strength development of OPC
mortar prisms and the effect of Bacillus cohnii on cement setting time and soundness. Microbial concentration of 1.0 × 10
7
cells·ml
−1
was used. Compressive strength tests analyses were carried out for each category of mortar prisms. Compressive
strength tests were carried out on the 2
nd
,7
th
,14
th
,28
th
,56
th
, and 90
th
day of curing in distilled water and microbial solutions. All
microbial mortars exhibited a greater compressive strength compared to the control with the highest observed at 90 days. Highest
percentage gain in compressive strength was observed at 90 days which is 28.3%. Microstructural analysis was carried out using a
scanning electron microscope (SEM) after 28 days of curing. e results indicated the presence of calcium carbonate and more
calcium silicate hydrate (CSH) deposits on the bacterial mortars. e bacteria did not have an effect on cement soundness. Setting
time was significantly accelerated.
1. Introduction
ere is an increasing need to enhance the durability and
sustainability of cement-made materials. ese materials are
expected to have a long service life of more than fifty years
[1]. However, due to degradation, this is sometimes im-
possible [2]. Due to this, there is a need for continuous
monitoring and repair of cement-made materials which is
expensive in the long run [3].
To a great extent, durability aspects of mortar/concrete
are possibly connected with its pore structure characteristics
[4]. Deleterious processes, such as rebar corrosion, caused by
ingress of chloride or carbonation, are aided by connectivity
within the pore system of the cement matrix. e vulner-
ability of these cement-based materials to the deleterious
processes is more if the pore structure is more open and
interconnected [5, 6]. Densification of the cement matrix
microstructure is one method of sealing the pores. is
increases the resistance of the material for ingress of the
deleterious materials such as CO
2
, chlorides, and sulphates
[7].
Ordinary Portland cement (OPC) exhibits shorter set-
ting times and achieves high compressive strength earlier (at
28 days of curing) compared to blended cements [8]. Due to
these properties, OPC is preferred in the construction of
dams and bridges in Kenya [8, 9]. OPC is, however, prone to
attack by deleterious substances such as chlorides and sul-
phates because of high amount of Ca(OH)
2
released as a
hydration product [8]. e pore structure of OPC is more
open compared to that of blended cement structures [9, 10].
Hence, there is a need for densification and refinement of its
microstructure. Cement-made structures using OPC have a
higher permeability due to their higher tendency to form
cracks. ese cracks tend to form a continuous pathway for
Hindawi
Journal of Chemistry
Volume 2020, Article ID 7816079, 8 pages
https://doi.org/10.1155/2020/7816079