Borneo Journal of Pharmacy http://journal.umpalangkaraya.ac.id/index.php/bjop/article/view/1070 Vol 2 Issue 2 November 2019 DOI: https://doi.org/10.33084/bjop.v2i2.1070 Page 114 – 118 e-ISSN: 2621-4814 INTRODUCTION Free radicals are atoms or molecules that have one or more unpaired electrons on their outer orbital, highly reactive. Increase the formation of free radicals ( Phaniendra et al ., 2015 ). Oxidative stress conditions would increase the formation of free radicals ( Lobo et al ., 2010). Highly reactive free radicals and oxygen species can initiate degenerative diseases such as cancer, diabetes, hypertension, atherosclerosis, and neurodegenerative disorders ( Liguori et al ., 2018). Antioxidants, molecules with a radical-scavenging capacity, are thought to exert a protective effect against free radical damage ( Lee et al ., 2017 ). In recent years, there has been an increasing interest in finding natural antioxidants, which can protect the human body from free radicals and retarded the progress of many chronic diseases ( Yashin et al ., 2017). Genus of Mangifera has been reported as an antioxidant activity with the DPPH scavenging method ( Ramirez et al ., 2014; Sultana et al ., 2012 ). The members of the Mangifera genus are used as food or traditional herbal medicine. Several members of Mangifera plants also have been used as an antioxidant, both traditionally used in the community and separated by the fractionation method to obtain the fraction with the highest antioxidant activity ( Lukmandaru et al ., 2012 ; Sutomo et al ., 2014 ). One of the medicinal plants from this genus was tandui ( Mangifera rufocostata Kosterm.). This plant was grown in South Kalimantan, Indonesia. Preliminary studies show that leaves of tandui contain tannins, phenolics, flavonoids, and steroidal saponins. Flavonoid and phenolic compounds may be useful as antioxidants from natural sources ( Tungmunnithum et al ., 2018 ). However, the antioxidant activity of tandui has not yet been Antioxidant Activity of Ethanolic Extract from Tandui Leaves (Mangifera rufocostata Kosterm.) by DPPH Radical Scavenging Method Revita Saputri * Tia Mariati Risma Melati Fitriyanti Department of Pharmacy, Sekolah Tinggi Ilmu Kesehatan Borneo Lestari, Banjarbaru, South Kalimantan, Indonesia * email: revita03@gmail.com Keywords: Antioxidant DPPH Ethanol extract Mangifera ruficostata Kosterm Tandui Abstract Tandui (Mangifera rufocostata Kosterm.) is a typical plant from South Kalimantan which belongs to the genus of Mangifera. Several species of Mangifera are known to have antioxidant activity. This study aimed to determine the antioxidant activity of ethanol extract of Tandui leaves. Tandui leaves that were obtained from the maceration method used 70% ethanol. Antioxidant activity was conducted quantitative using the DPPH (2,2-diphenyl-1-picrylhydrazil) method. The result of the antioxidant activity of Ethanol extract of Tandui leaves quantitatively obtained IC50 value was 60.7042 µg/mL. The ethanol extract of Tandui leaves has strong antioxidant activity. Received: October 17 th 2019 Accepted: October 31 th 2019 Published: November 14 th 2019 © 2019 Revita Saputri, Tia Mariati Risma Melati, Fitriyanti. Published by Institute for Research and Community Services Universitas Muhammadiyah Palangkaraya. This is an Open Access article under the CC-BY-SA License (http://creativecommons.org/licenses/by-sa/4.0/). DOI: https://doi.org/10.33084/bjop.v2i2.1070.