_____________________________________________________________________________________________________ *Corresponding author: E-mail: Ojoye.briggs@ust.edu.ng; Journal of Complementary and Alternative Medical Research 9(3): 51-57, 2020; Article no.JOCAMR.57368 ISSN: 2456-6276 Anti-hyperglycaemic, Anti-dyslipidaemic and Hepatoprotective Effects of the Polyherbal Mixture Diarth in Alloxan-induced Diabetic Rats Ojoye N. Briggs 1* , Kemzi N. Elechi-Amadi 1 , Chrissie Okobia 1 and Ferdinand C. Ezeiruaku 2 1 Department of Medical Laboratory Science, Rivers State University, Nkpolu-Oroworukwo, Port Harcourt, Nigeria. 2 Department of Medical Laboratory Science, Niger Delta University, Wilberforce Island, Bayelsa State, Nigeria. Authors’ contribution This work was carried out in collaboration among all authors. Author ONB designed the study, performed the statistical analysis and wrote the first draft of the manuscript. Authors KNEA and CO wrote the protocol and managed the literature searches. Author FCE managed the analyses of the study. All authors read and approved the final manuscript. Article Information DOI: 10.9734/JOCAMR/2020/v9i330145 Editor(s): (1) Assist. Prof. Suma B. V., Ramaiah University of Applied Sciences, India. Reviewers: (1) Shailja Puri, India. (2) Dashputre Neelam Laxman, Savitribai Phule Pune University, India. Complete Peer review History: http://www.sdiarticle4.com/review-history/57368 Received 09 March 2020 Accepted 14 May 2020 Published 26 May 2020 ABSTRACT Aim: This study evaluates the anti-hyperglycaemic, anti-dyslipidaemic and hepatoprotective effects of the polyherbal mixture diarth, in alloxan-induced diabetic rats. Methodology: A total of 35 male Wistar albino rats weighing between 120-140 g were used for this study. Diabetes was induced by a single intraperitoneal injection of freshly prepared alloxan- monohydrate (140 mg/kg body weight). Fasting plasma glucose (FPG) was determined using the glucose oxidase method. Total Cholesterol (TC), Triglyceride (TG) and High density lipoprotein cholesterol (HDL-C) were determined using enzymatic methods. Low density lipoprotein cholesterol (LDL-C) was calculated using the Friedewald’s equation. Alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were determined using Reitman-Frankel method, Original Research Article