S342 Abstracts / Toxicology Letters 196S (2010) S37–S351 using the Optimized Monier-Williams method. The type and quan- tity of benzoic acid and sorbic acid were determined by reverse phase liquid chromatography. Sulfur dioxide was found in 39 of 100 samples at the level ranging from 11.01 to 2106.94 mg/kg. The mean ± SD of sulfur dioxide was 377.29 ± 456.68 mg/kg. Benzoic acid was found in 10 samples ranging from 31.37 to 1384.45 mg/kg. The mean ± SD of benzoic acid was 286.608 ± 415.68 mg/kg. Two coconut sap samples were found to contain both sulfur dioxide and benzoic acid. Therefore, the potential misuse of these preser- vatives should be strictly controlled and they should be suitably used in order to improve the safety of products made from coconut sap. Otherwise the production might need to be controlled by food sanitation and promotion of use of natural preservatives. doi:10.1016/j.toxlet.2010.03.1079 P309-046 Nitrate, lead and cadmium contents in beans, leafy, fruit and root vegetables from conventional, good agricultural practice and organic cultivations W. Karnpanit, W. Benjapong, S. Srianujata, N. Dhananiveskul, A. Poowanasatien, C. Jatutipsompol Institute of Nutrition, Mahidol University, Thailand This study aimed to determine the nitrate, lead and cadmium contents in different types of vegetables from conventional, good agricultural practice and organic cultivations and to estimate nitrate, lead and cadmium intakes from vegetable consumption. Nitrate contents in edible part of vegetables were determined by the cadmium reduction method and colorimetric method while lead and cadmium contents were determined by Graphite Fur- nace Atomic Absorption Spectrometry (GFAAS). This study found that leafy vegetables contained higher levels of nitrate than that of beans, fruit, and root vegetables. The different families of vegeta- bles had different levels of nitrate. The Cruciferae family contained nitrate content higher than the others. The different levels of nitrate were found in each kind of vegetables because of the different fac- tors in cultivation such as types of vegetables, types and amount of fertilizers, types of soil and light intensity. It was found in this study that the application of large amounts of fertilizers, especially nitrogen fertilizers was the major factor that caused high levels of nitrate residues in vegetables. For lead and cadmium contents, the study found that most of the leafy vegetables contained lead and cadmium levels higher than that of beans, fruit and root vegeta- bles. It was also found that lead and cadmium levels in most of the leafy vegetables from conventional and good agricultural practice cultivations were higher than in the organic cultivation. The study indicated that besides vegetable types, different levels of lead and cadmium in the same type of vegetable may depend on the different cultivation area. The important factor in lead and cadmium contam- ination comes from the environment around the farm. The study of nitrate, lead and cadmium intake indicated that the average intakes of nitrate, lead and cadmium from vegetable consumption in Thai population were lower than the acceptable intake levels. doi:10.1016/j.toxlet.2010.03.1080 P309-047 Simultaneous determination of eight biogenic amines in fish by liquid chromatography with fluorescence detector and tandem mass spectrometry M. Fernández-Franzón 1 , F. De Berardinis 2 , G. Sagratini 2 , S. Vittori 2 , G. Font 1 , J. Ma ˜ nes 1 1 Laboratory of Food Chemistry and Toxicology, Faculty of Pharmacy, University of Valencia, Spain, 2 Dipartimento di Scienze Chimiche, Facoltà di Farmacia, Università degli Studi di Camerino, Italy Biogenic amines are organic compounds naturally form in food from the decarboxylation of amino acids by enzymatic reactions. The composition in biogenic amines gives information about the hygienic conditions of raw material and manufacturing practices since their accumulation is associated with bacterial contamina- tion and spoilage during storage. Biogenic amines may be present in various foods, particularly fish and fish products, cheese, meat and fermented foods. The aim of this study was to compare two analytical methods developed for the analysis of eight biogenic amines (Tryptamine, Spermine, Spermidine, Histamine, Tyramine, Putrescine, Cadaver- ine, Phenylethylamine) in fish. These two analytical methods are liquid chromatography and fluorescence detector with precolumn derivatization using dansyl chloride and liquid chromatography coupled to a tandem mass spectrometry with a triple quadrupole (QqQ) analyzer without derivatization process. The major drawbacks in both analyses are represented by the relevant matrix effect and the presence of an unknown amount of amines in fish that difficulties the use of a blank sample for valida- tion studies. In both analyses the method of standard addition was used for quantification. Liquid chromatography coupled to tandem mass spectrometry provides higher sensitivity (0.025 mg kg -1 for Phenylethylamine to 0.5 mg kg -1 for Spermine) when compared to fluorescence detection (0.5 mg kg -1 for Putrescine to 20 mg kg -1 for Histamine) and also showed to be more precise. The applicabil- ity of the method to the determination of biogenic amines in fish products from commercials of Valencia was demonstrated. doi:10.1016/j.toxlet.2010.03.1081 P309-048 Deoxynivalenol and fusarium species presence in raw materials for animal feed F. Caloni 1 , C. Cortinovis 1 , M. Pasquali 2 , F. Giraud 2 , A.C. Gutleb 2 1 Università degli Studi di Milano, Department of Veterinary Sciences and Technologies for Food Safety Via Celoria 10 20133 Milan, Italy, 2 Centre de Recherche Public – Gabriel Lippmann, Belvaux, Luxembourg Deoxynivalenol (DON), a tricothecene mycotoxin produced by moulds of Fusarium, especially F. graminearum, is a natural con- taminant that occurs throughout the world in cereal grains and feedstuffs. DON exposure is generally associated with emesis, feed refusal, depressed feed intake, and possibly impaired immune func- tion in all animal species. In this study the presence of DON in raw materials for animal feed, collected from January 2008 to December 2009, was evaluated. A total of 106 samples, including corn (40), wheat (35), soya (25), sorghum (5), and sunflower flour (1) were analyzed with a specific ELISA test, commercially available.