ABSTRACTS POSTER PRESENTATIONS BURNS PP-001 SNP and mtDNA Analysis by PLEX-ID Assay in Maternity Testing When the False Mother Can’t be Excluded by 46 STRs Genotyping Li Li 1 , Suhua Zhang 1 , Yuan Lin 1 , Yan Liu 1 , Chengtao Li 1 , Shumin Zhao 1 1 Shanghai Key Laboratory of Forensic Medicine, Institute of Forensic Science, Ministry of Justice, China 2 Research and Development, Ibis Biosciences Inc., A Subsidiary of Abbott Molecular, Inc Objective: To reach an accurate conclusion, mtDNA and SNP analysis by PLEX-ID assay was applied to an extreme maternity case where the false mother and the child have at least one allele shared at autosomal 46 STR loci, giving inclusion of maternity with maternity indices of 3.3E+13. Method: Genomic DNA was extracted using the Chelex-100 and proteinase K protocol from blood samples of the child and the adopting woman. The quantity of recovered DNA was determined by a spectro- photometric method. 19 autosomal STR loci plus Amelogenin were amplified using the AmpFlSTR® SinofilerTM kit (Life Technologies, Carlsbad, CA) and PowerPlex®16 System (Promega, USA) following the manufacturers’ recommendations. An additional 27 autosomal STR loci plus Amelogenin were analyzed using two domestic kits AGCU 21+1 (http://www.agcu.cn/) and STRtyper-10 G (http://www.zhcodon. com/). Locus D19S433 was included in SinofilerTM kit and the domestic kit 21+1. Amplification reactions were carried out using the GeneAmp PCR system 9700 (Life Technologies). The amplified products were analyzed using 3130xl Genetic Analyzer (Life Technologies). Genotyp- ing data were determined by GeneMapper v3.2.1 software. Additional assay was carried out that genotypes 40 ideal forensic autosomal SNPs (heterozygosity is near 0.5) identified in Kenneth Kidd’ s lab at Yale. Besides, mtDNA assay was applied that has 24 primer pairs in 8 triplex reactions for HVIand HVII. Results: The alleged mother and the boy shared at least one allele at all 46 tested autosomal STR loci which yielded a very high maternity index between them. But, according to the SNP profile data for 40 autosomal SNP markers, different homozygous genotypes between the alleged mother and son at five exclusionary loci (rs7229946, rs985492, rs9951171, rs214955, rs1109037) excluded maternity. Mi- tochondrial profiles also clearly exclude mother as a parent of the alleged son because the two persons have multiple differences(i.e. the base compositions are not the same at the fragments 16102-16224nt, 16130–16224 nt,16154-16268 nt,16231-16338 nt,16256-16366 nt,16318-16402 nt in HV-I and 83–187 nt,113-245 nt,204-330 nt,239-363 nt,239-363,262-390nt in HV-II. Conclusions: Different kinds of genetic markers are needed to supple- ment the use of autosomal STR loci in case where the alleged parent is suspected to be related to the true parent. In the case herein, ESI-TOF- MS SNP and mtDNA analysis using the PLEX-ID platform is more discriminating than STR genotyping by CGE method. PP-002 The Retrospective Analysis of 15 Burned Cases Caused by Laser and Solarium(Tanning Booths) in Beauty-Medical Centers Evaluated Between 2006–2010 in Istanbul Council of Forensic Medicine 3rd Specialization Board Süheyla F. Aliustaoğlu 1 , Harun Akkaya 2 , Seyfullah Şahin 3 , Erdinç Özdemir 3 , C. Haluk İnce 4 1 Council of Forensic Medicine, 4th Specialty Board, Istanbul, Turkey. 2 Council of Forensic Medicine, 2nd Specialty Board, Istanbul, Turkey. 3 Council of Forensic Medicine, Istanbul, Turkey. 4 President of Council of Forensic Medicine, Istanbul, Turkey. Introduction: It is a well known fact that since old times people are making efforts for their physical appearance. With significant progress in technology, laser epilations and tanning booths etc. (radiation and UV rays) became helpful aesthetically, making an impact in a very shortest time. Nowadays in large-scaled beauty-medical centers nearly all kind of laser and tanning booths can be seen. Since these machines are medical, they need to be used by doctors or medical staff. Methods: Between January 2006 and December 2010, the demographic data of 15 people analysed retrospectively in this study whose physical examination held in Council of Forensic Medicine 3rd Specialization Board, who applied to beauty-medical centers for laser epilation, to erase tattoo, get tan in solarium (tanning booth) and as a consequence burn scars arose and they brought a suit against those centers. Results: The average age is 32.13±8.2 of the total 15 people, 12 (80 % ) of them is female. 10 of them brought suit for the bad consequences of laser epilation, 1 for needle electric epilation, 2 for erasing tattoo and 2 for getting burn in tanning booths. 7 (46.6 %) of 15 people were assessed in 2010. 2 people got burnt in tanning booth had 2nd degree of massive burnt; one of them died of burnt complications. 10 people who applied laser epilation had burnt scars and hiperpigmented lesions; 2 people who applied tattoo erasure by laser had burnt scars and cheloid tissue. 7 people stated they were not informed sufficiently, not any informed consent found in the dossier. In the reports of Council of Forensic Medicine; 9 (60 %) of 15 cases occurred by wrong medical application without any surveillance of experts. Conclusion: The utilization of those devices increasing in a very short time by non-medical or uncertified people is forbidden. Within the frame of laser applications; different laser rays should vary according to the skin This Supplement was not sponsored by any outside commercial interests. Int J Legal Med (2012) 126 (Suppl 1):S121–S384 DOI 10.1007/s00414-012-0711-9