In our previous TiPS Web Watch article on G-protein-coupled receptor (GPCR) websites [1], we provided information about several websites related to GPCRs that are available in the public domain. Our goal was to guide interested readers towards web-based computational tools that are specifically designed for genomic and structural studies of GPCRs, in addition to databases with GPCR sequence information or with experimental data to help identify responses to agonists and antagonists. Since the publication of this article, several new GPCR websites have emerged and existing websites have released new versions that contain additional tools (Table 1). This is not surprising because research on GPCRs is very active, in part because the GPCR superfamily represents the largest class of receptors in mammalian genomes, and receptors in this superfamily are current or proposed targets of a large number of drugs. In this article, we provide an update of existing GPCR websites and describe new tools not reviewed in our previous article. We also include other general websites that might prove useful for those interested in GPCRs. GPCR-dedicated websites The most comprehensive GPCR database tool currently available in the public domain is the G Protein- Coupled Database (GPCRDB; http://www.gpcr.org). GPCRDB integrates sequence data, evolutionary relationships and pharmacological data [2]. Two new features in the 2002 release 6.0 of GPCRDB are: (1) a ‘BLAST’ service in which users can query a sequence against the GPCRDB; and (2) an additional source of ligand binding data. In the coming months, further data will be integrated into GPCRDB, including point mutation data directly extracted from the literature with the option to display point mutations from TinyGrap (http://tinygrap.uit.no/) or deduced from published work along with multiple sequences alignments. Links to the Gene Ontology data and cross- references to the IUPHAR Receptor database, which is due to be released in December 2002, will also become available (http://www.iuphar.org/). This database will contain detailed information about GPCRs (ultimately for ~350 non-sensory GPCRs) and other receptors (including ion channel and ligand-gated channel receptors, enzyme-associated receptors, and transcriptional factor receptors), and will be searchable and addressable via a newly devised system of receptor codes. In addition to the Olfactory Receptor Database and its sister database the Odorant Molecule Database described previously [1], several new databases dedicated to specific subfamilies of GPCRs and their ligands and pathways are available. The Chemokine Receptor Database (http://crf.medic.kumamoto-u.ac.jp/ CSP/Receptor.html) provides genomic and protein links for all known and putative chemokine receptors. This database is a subset of the larger database Cytokine Signaling Pathway Database (http://crf.medic.kumamoto-u.ac.jp/), which contains biochemical and sequence data and references regarding signaling molecules (e.g. receptors, ligands and kinases, among others) and their TRENDS in Pharmacological Sciences Vol.23 No.11 November 2002 http://tips.trends.com 0165-6147/02/$ – see front matter © 2002 Elsevier Science Ltd. All rights reserved. PII: S0165-6147(02)02113-2 535 Forum Web Watch G-protein-coupled receptor websites Brinda K. Rana and Paul A. Insel Table 1. G-protein-coupled receptor (GPCR) and other receptor websites Website URL Date last updated or released GPCR-specific websites Chemokine Receptor http://crf.medic.kumamoto-u.ac.jp/CSP/ 20 February 2001 Family Database Receptor.html Luteinizing Hormone http://www.eur.nl/fgg/endov/lhr.html 11 April 2001 Receptor Mutation DB Cytokine Signaling http://crf.medic.kumamoto-u.ac.jp/ 4 July 2001 Pathway Database GPCRDB (Version 6) http://www.gpcr.org 8 March 2002 Visuer http://transport.physbio.mssm.edu/viseur/ 14 December viseur.html 1999 Olfactory Database http://senselab.med.yale.edu/senselab/ June 1999 (Version 4) ORDB/ TinyGrap (Version 10) http://tinygrap.uit.no/ April 2001 WNT gene Homepage http://www.stanford.edu/~rnusse/ April 2002 wntwindow.html The Swiss-Model 7TM http://www.expasy.org/swissmod/ SWISS-MODEL.html GPCR Pattern Recognition http://www.biochem.ucl.ac.uk/bsm/ dbbrowser/GPCR/ IUPHAR Receptor http://www.iuphar.org/ To be released Database December 2002 Endogenous GPCR List http://www.biomedcomp.com/GPCR.html 13 February 1999 Other websites Pharmacological Targets http://research.bmn.com/ptbase September 2002 Database (PTbase) Homophila http://homophila.sdsc.edu/ March 2002 SBASE (Release 9) http://hydra.icgeb.trieste.it/sbase/ 13 June 2002 HGVbase (Release 13.0) http://hgvbase.cgb.ki.se/ 27 March 2002 JSNP (Release 11) http://snp.ims.u-tokyo.ac.jp/ 17 July 2002 The SNP Consortium Ltd http://snp.cshl.org/ Regularly updated Entrez SNP http://www.ncbi.nlm.nih.gov/entrez/query. Regularly fcgi?db snp updated GenMapp http://www.genmapp.org/public/PNAS_Ro1/ Gprotein.asp PDSP http://pdsp.cwru.edu/ PharmGKB Community http://www.pharmgkb.org/community/ July 2002 Project search.jsp