© CSIRO 2017 Marine and Freshwater Research, 2017, 68, 922–930 http://dx.doi.org/10.1071/MF16026_AC Page 1 of 3 Supplementary material Bioaccumulation, oxidative stress and cellular damage in the intertidal gastropod Bembicium nanum exposed to a metal contamination gradient Rodney P. Ubrihien A,B , Anne M. Taylor A and William A. Maher A A Ecochemistry Laboratory, Institute for Applied Ecology, University of Canberra, University Drive, Bruce, ACT 2617, Australia. B Corresponding author. Email: rod.ubrihien@canberra.edu.au Table S1. Measured values and certified reference values (μg g –1 dry mass) for certified reference material 1556b samples that were digested and analysed with metal samples Element Measured value Certified value Mn 17 ± 1 18.5 ± 0.2 Fe 178 ± 15 206 ± 7 Cu 66 ± 5 72 ± 2 Zn 1285 ± 122 1424 ± 46 As 7.1 ± 0.4 7.65 ± 0.65 Cd 2.4 ± 0.3 2.48 ± 0.08 Table S2. Metal concentrations (μg g –1 dry mass) in B. nanum whole tissue for organisms sampled at Port Kembla, Shellharbour and Kiama Mean and standard error values are calculated from combined data for organisms sampled September 2010, November 2011, January 2012 and March 2012 (n = 22) (% mol indicates the mean percentage of the total molar concentration of the metal burden for the site) Port Kembla Shellharbour Kiama Mean ± s.e. % mol Mean ± s.e. % mol Mean ± s.e. % mol Mn 86 ± 10 6.6 59 ± 13 11.8 41 ± 8 8.9 Fe 351 ± 38 26.5 251 ± 32 48.9 169 ± 20 36.1 Cu 839 ± 83 55.7 71 ± 11 12.1 152 ± 23 28.5 Zn 148 ± 10 9.5 128 ± 6 21.2 125 ± 11 22.8 As 30 ± 4 1.7 40 ± 9 5.9 22 ± 2 3.5 Cd 1.21 ± 0.24 0 1.61 ± 0.25 0.1 1.75 ± 0.24 0.2