ISSN 0976 – 9080 BIOMIRROR - An Open Access Journal www.bmjournal.in BM/Vol.1/December 2010/bm-1111251610 Homology Modeling and Enzyme Function Prediction in the Hypothetical Proteins of Helicobacter pylori - an Insilico Approach D G Gore 1 *, A P Denge 1 and N M Amrute 1 1 * Sai Bioinfosys- Bioinformatics Research Centre, Raghuji Nagar, Nagpur 440023 India. 1 BIOMIRROR Volume 1(7) :1-5 (2010) ISSN 0976 – 9080 BM, an open access journal Abstract Severity of Helicobacter pylori (hpy) for human is a present problem and asks for better research to understand the mechanism of pathogenecity and drug resistance. In view, genome data of hpy highlighted, major regions codes for hypothetical proteins and could be getting involved in the severity of hpy. In decision, web tools like CDD BLAST, INTERPROSCAN, PFAM and COGs have been implemented to search enzyme coding ability in these regions along with PS square structure prediction server for tertiary structure modeling. Study sorted 179 h. proteins to code for specific enzyme based on conserved domain search by programs and 123 structures were built using prediction server. Study highlighted and filtered the uncharacterized regions of hpy possessing ability to code enzyme. Keywords: Helicobacter pylori, conserved domain, enzyme function, structure prediction, web tools, and bioinformatics. Introduction Helicobacter pylori (hpy) is human and primate pathogen colonizes gastric lumen and produces severe diseased conditions. Hpy is microaerophilic spiral-shaped lophotrichous Gram-negative bacterium [1]. H. pylori was reported to cause of chronic active gastritis and peptic ulcer disease in humans and linked to be a causative agent for the development of gastric adenocarcinoma and MALT lymphoma [2, 3, 4]. Several isolates from peptic ulcer disease and non ulcer dyspepsia patients were reported resistant to clarithromycin, amoxicillin and metronidazole. [5].The circular genome of hpy consist of 1667867 nucleotides and codes for 1573 ORFs for protein and 43 ORFs for RNA. Out of these, 627 marked for the hypothetical proteins whose exact functions in the lifecycle of hpy were not known [6]. * Corresponding author: D G Gore 1 * Sai Bioinfosys- Bioinformatics Research Centre, Raghuji Nagar, Nagpur 440023 India. Email: saibioinfosys@gmail.com Received November 24, 2010; Accepted December 09, 2010; Published December 16, 2010 Citation: D G Gore 1 *, A P Denge 1 and N M Amrute 1 (2010-Homology Modeling and Enzyme Function Prediction in the Hypothetical Proteins of Helicobacter pylori - an Insilico Approach) BIOMIRROR : 1-5/ bm- 1111251610 Copyright: © 2010 D G Gore*, et al. This is an open- access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited