*Correspondence: art.mbio@patnawomenscollege.in (Received: March 16, 2021; accepted: April 22, 2021) Citaton: Kumari A, Kumar P, Kumar M, Kumar J. In silico Analysis of Forskolin as a Potental Inhibitor of SARS-CoV-2. J Pure Appl Microbiol. 2021;15(2):709-715. doi: 10.22207/JPAM.15.2.22 © The Author(s) 2021. Open Access. This artcle is distributed under the terms of the Creatve Commons Atributon 4.0 Internatonal License which permits unrestricted use, sharing, distributon, and reproducton in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creatve Commons license, and indicate if changes were made. Kumari et al. | J Pure Appl Microbiol | 15(2):709-715 | June 2021 Artcle 6933 | htps://doi.org/10.22207/JPAM.15.2.22 Print ISSN: 0973-7510; E-ISSN: 2581-690X ReseARCh ARtiCle OPeN ACCess www.microbiologyjournal.org 709 Journal of Pure and Applied Microbiology In silico Analysis of Forskolin as a Potental Inhibitor of sARs-CoV-2 Art Kumari 1 *, Prashant Kumar 2 , Manindra Kumar 3  and Jainendra Kumar 4  1 Department of Microbiology, Patna Women's College (Autonomous) Patna University, Patna, Bihar - 800001, India. 2 Department of Zoology, Ramjaipal College, Chapra, Bihar, India. 3 Former Faculty member, Insttute of Modern Biology & Applied Sciences, Patna, Bihar, India. 4 Former Professor, Department of Botany & Biotechnology, Patliputra University, Patna, Bihar, India. Abstract Coronavirus disease 2019 (COVID-19) has spread rapidly as global pandemic afectng 187 countries/ regions and emerged as worldwide health crisis. Potental antviral drugs used for the SARS-CoV-2 in clinical treatments have side efects. However, emergency vaccines are in use but despite that increase in the coronavirus cases are alarming. Thus, it is utmost need of safer antviral agent to treat or inhibit the viral infecton. Forskolin has been reported as a possible antviral-agent. This molecule was docked with ACE2 receptor of human which is the target for the binding of S1 unit of viral S protein of SARS- CoV-2. In silico docking was carried out on SwissDock, PatchDock and FireDock servers. The docked ACE2 structure was further docked with the RBD of the spike protein. Forskolin is able to H-bond with the hACE2 and ACE2-forskolin fails to interact with the receptor-binding domain (RBD) of the Spike protein of SARS-CoV-2. Instead, viral RBD is repulsed by the diterpene molecule through obliteraton and reciprocated binding. We report frst that forskolin plays a crucial role in the inhibiton of protein- protein interacton of RBD and ACE2 when docked with either of the protein. Keywords: SARS-CoV-2, S protein RBD, human ACE2 receptor, forskolin, Plectranthus barbatus, Coleus forskohlii